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Investigation of the changes of biophysical/mechanical characteristics of differentiating preosteoblasts in vitro

Overview of attention for article published in Biomaterials Research, November 2015
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Title
Investigation of the changes of biophysical/mechanical characteristics of differentiating preosteoblasts in vitro
Published in
Biomaterials Research, November 2015
DOI 10.1186/s40824-015-0046-y
Pubmed ID
Authors

Ramesh Subbiah, Muhammad Suhaeri, Mintai P Hwang, Woojun Kim, Kwideok Park

Abstract

Topography, stiffness, and composition of biomaterials play a crucial role in cell behaviors. In this study, we have investigated biochemical (gene markers), biophysical (roughness), and biomechanical (stiffness) changes during the osteogenic differentiation of preosteoblasts on gelatin matrices. Our results demonstrate that gelatin matrices offer a favorable microenvironment for preosteoblasts as determined by focal adhesion and filopodia formation. The osteogenic differentiation potential of preosteoblasts on gelatin matrices is confirmed by qualitative (Alizarin red, von kossa staining, immunofluorescence, and gene expression) and quantitative analyses (alkaline phosphatase activity and calcium content). The biomechanical and biophysical properties of differentiating preosteoblasts are analyzed using atomic force microscopy (AFM) and micro indentation. The results show sequential and significant increases in preosteoblasts roughness and stiffness during osteogenic differentiation, both of which are directly proportional to the progress of osteogenesis. Cell proliferation, height, and spreading area seem to have no direct correlation with differentiation; however, they may be indirectly related to osteogenesis. The increased stiffness and roughness is attributed to the mineralized bone matrix and enhanced osteogenic extracellular matrix protein. This report indicates that biophysical and biomechanical aspects during in vitro cellular/extracellular changes can be used as biomarkers for the analysis of cell differentiation.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 25%
Student > Master 3 25%
Researcher 2 17%
Student > Bachelor 2 17%
Other 1 8%
Other 0 0%
Unknown 1 8%
Readers by discipline Count As %
Engineering 5 42%
Materials Science 2 17%
Biochemistry, Genetics and Molecular Biology 2 17%
Medicine and Dentistry 1 8%
Unknown 2 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 November 2015.
All research outputs
#5,611,513
of 6,577,121 outputs
Outputs from Biomaterials Research
#17
of 22 outputs
Outputs of similar age
#196,040
of 247,791 outputs
Outputs of similar age from Biomaterials Research
#6
of 10 outputs
Altmetric has tracked 6,577,121 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 22 research outputs from this source. They receive a mean Attention Score of 2.8. This one scored the same or higher as 5 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 247,791 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.