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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS
|
|---|---|
| Published in |
BMC Biotechnology, November 2015
|
| DOI | 10.1186/s12896-015-0222-0 |
| Pubmed ID | |
| Authors |
Thomas McDonnell, Charis Pericleous, Emmanuelle Laurine, Rita Tommasi, Acely Garza-Garcia, Ian Giles, Yiannis Ioannou, Anisur Rahman |
| Abstract |
In this paper we describe a novel method to achieve high yield bacterial expression of a small protein domain with considerable therapeutic potential; Domain I of Beta-2-glycoprotein I (β2GPI). β2GPI is intrinsic to the pathological progression of the Antiphospholipid Syndrome (APS). Patients develop autoantibodies targeting an epitope located on the N-terminal Domain I of β2GPI rendering this domain of interest as a possible therapeutic. This new method of production of Domain I of β2GPI has increased the production yield by ~20 fold compared to previous methods in E.coli. This largely scalable, partially automated method produces 50-75 mg of pure, folded, active Domain I of β2GPI per litre of expression media. The application of this method may enable production of Domain I on sufficient scale to allow its use as a therapeutic. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 13% |
| Student > Doctoral Student | 2 | 13% |
| Student > Master | 2 | 13% |
| Researcher | 2 | 13% |
| Student > Ph. D. Student | 1 | 7% |
| Other | 2 | 13% |
| Unknown | 4 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 33% |
| Agricultural and Biological Sciences | 2 | 13% |
| Medicine and Dentistry | 2 | 13% |
| Immunology and Microbiology | 1 | 7% |
| Neuroscience | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 4 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 April 2017.
All research outputs
#2,943,943
of 22,833,393 outputs
Outputs from BMC Biotechnology
#119
of 935 outputs
Outputs of similar age
#44,147
of 281,503 outputs
Outputs of similar age from BMC Biotechnology
#5
of 22 outputs
Altmetric has tracked 22,833,393 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 935 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,503 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.