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Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements

Overview of attention for article published in Genome Biology, November 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (94th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (58th percentile)

Mentioned by

blogs
2 blogs
twitter
16 X users
patent
7 patents

Citations

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184 Dimensions

Readers on

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366 Mendeley
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Title
Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
Published in
Genome Biology, November 2015
DOI 10.1186/s13059-015-0818-7
Pubmed ID
Authors

Tautvydas Karvelis, Giedrius Gasiunas, Joshua Young, Greta Bigelyte, Arunas Silanskas, Mark Cigan, Virginijus Siksnys

Abstract

To expand the repertoire of Cas9s available for genome targeting, we present a new in vitro method for the simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements. The method relies on the in vitro cleavage of plasmid libraries containing a randomized PAM as a function of Cas9-guide RNA complex concentration. Using this method, we accurately reproduce the canonical PAM preferences for Streptococcus pyogenes, Streptococcus thermophilus CRISPR3 (Sth3), and CRISPR1 (Sth1). Additionally, PAM and sgRNA solutions for a novel Cas9 protein from Brevibacillus laterosporus are provided by the assay and are demonstrated to support functional activity in vitro and in plants.

X Demographics

X Demographics

The data shown below were collected from the profiles of 16 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 366 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 <1%
Korea, Republic of 1 <1%
Switzerland 1 <1%
China 1 <1%
Belgium 1 <1%
Unknown 359 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 68 19%
Researcher 63 17%
Student > Bachelor 57 16%
Student > Master 48 13%
Student > Doctoral Student 15 4%
Other 37 10%
Unknown 78 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 130 36%
Agricultural and Biological Sciences 104 28%
Immunology and Microbiology 11 3%
Chemical Engineering 7 2%
Engineering 7 2%
Other 20 5%
Unknown 87 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 29. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 November 2023.
All research outputs
#1,355,656
of 25,373,627 outputs
Outputs from Genome Biology
#1,064
of 4,467 outputs
Outputs of similar age
#22,269
of 392,489 outputs
Outputs of similar age from Genome Biology
#31
of 75 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,467 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 392,489 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 75 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.