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Identification of a Golgi-localized UDP-N-acetylglucosamine transporter in Trypanosoma cruzi

Overview of attention for article published in BMC Microbiology, November 2015
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3 tweeters

Citations

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5 Dimensions

Readers on

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19 Mendeley
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Title
Identification of a Golgi-localized UDP-N-acetylglucosamine transporter in Trypanosoma cruzi
Published in
BMC Microbiology, November 2015
DOI 10.1186/s12866-015-0601-7
Pubmed ID
Authors

Carlos Gustavo Baptista, Elizabeth Cristina Rodrigues, Patricia Morking, Amanda Klinke, Maria Luiza Zardo, Maurílio José Soares, Alessandra Melo de Aguiar, Samuel Goldenberg, Augusto Savio Peixoto Ramos

Abstract

Nucleotide sugar transporters (NSTs) play an essential role in translocating nucleotide sugars into the lumen of the endoplasmic reticulum and Golgi apparatus to be used as substrates in glycosylation reactions. This intracellular transport is an essential step in the biosynthesis of glycoconjugates. We have identified a family of 11 putative NSTs in Trypanosoma cruzi, the etiological agent of Chagas' disease. A UDP-N-acetylglucosamine transporter, TcNST1, was identified by a yeast complementation approach. Based on a phylogenetic analysis four candidate genes were selected and used for complementation assays in a Kluyveromyces lactis mutant strain. The transporter is likely expressed in all stages of the parasite life cycle and during differentiation of epimastigotes to infective metacyclics. Immunofluorescence analyses of a GFP-TcNST1 fusion protein indicate that the transporter is localized to the Golgi apparatus. As many NSTs are multisubstrate transporters, we also tested the capacity of TcNST1 to transport GDP-Man. We have identified a UDP-N-acetylglucosamine transporter in T. cruzi, which is specifically localized to the Golgi apparatus and seems to be expressed, at the mRNA level, throughout the parasite life cycle. Functional studies of TcNST1 will be important to unravel the role of NSTs and specific glycoconjugates in T. cruzi survival and infectivity.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 5%
Germany 1 5%
Unknown 17 89%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 42%
Student > Master 3 16%
Student > Ph. D. Student 2 11%
Other 1 5%
Student > Bachelor 1 5%
Other 2 11%
Unknown 2 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 53%
Biochemistry, Genetics and Molecular Biology 3 16%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Immunology and Microbiology 1 5%
Chemistry 1 5%
Other 1 5%
Unknown 2 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 November 2015.
All research outputs
#4,635,939
of 6,593,220 outputs
Outputs from BMC Microbiology
#753
of 1,207 outputs
Outputs of similar age
#156,878
of 248,547 outputs
Outputs of similar age from BMC Microbiology
#34
of 76 outputs
Altmetric has tracked 6,593,220 research outputs across all sources so far. This one is in the 26th percentile – i.e., 26% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,207 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 248,547 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 76 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.