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Rapid detection of the common avian leukosis virus subgroups by real-time loop-mediated isothermal amplification

Overview of attention for article published in Virology Journal, November 2015
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Title
Rapid detection of the common avian leukosis virus subgroups by real-time loop-mediated isothermal amplification
Published in
Virology Journal, November 2015
DOI 10.1186/s12985-015-0430-1
Pubmed ID
Authors

Hao Peng, Lili Qin, Yuyu Bi, Peikun Wang, Guangzhen Zou, Jun Li, Yongli Yang, Xingfu Zhong, Ping Wei

Abstract

Subgroups A, B, E and J are the major subgroups of avian leukosis virus (ALV) infecting chickens. ALV infection has become endemic in China and has a significant negative effect on the poultry industry. Consequently, there is an urgent need for a specific, sensitive and rapid method for diagnosis and eradication of ALV. Therefore, we developed a simple and rapid real-time loop-mediated isothermal amplification (LAMP) reaction for the timely detection of the common ALV subgroups, whereby the amplification can be obtained in 35 min under isothermal conditions at 63 °C, ability to specific, sensitive and rapid detect all the common ALV subgroups. A set of four specific primers was designed to target the sequences of the pol gene of ALV, and the loop-mediated isothermal amplification (LAMP) assay were developed and compared with PCR and virus isolation methods. The results from specificity of the LAMP assay showed that only target ALVs DNA was amplified. The LAMP assay demonstrated a sensitivity of 20 copies/reaction of ALV DNA, which was 10 times higher than the conventional PCR measurement. To further evaluate the reliability of the method, the assay was evaluated with ALV DNA from a panel of 81 clinical samples suspected of ALV infection. The results verify that the LAMP method was more sensitive than the conventional PCR and virus isolation method. In conclusion, the developed LAMP assay was a simple, inexpensive, sensitive method for the rapid detection of the most common subgroups of ALV, and it provided a useful and practical tool in the eradication program for ALV in the poultry industry.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 13%
Researcher 2 13%
Student > Master 2 13%
Student > Bachelor 1 7%
Lecturer > Senior Lecturer 1 7%
Other 1 7%
Unknown 6 40%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 27%
Biochemistry, Genetics and Molecular Biology 3 20%
Computer Science 1 7%
Chemistry 1 7%
Unknown 6 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 November 2015.
All research outputs
#15,350,522
of 22,833,393 outputs
Outputs from Virology Journal
#1,960
of 3,044 outputs
Outputs of similar age
#226,602
of 386,693 outputs
Outputs of similar age from Virology Journal
#36
of 52 outputs
Altmetric has tracked 22,833,393 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,044 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.8. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 386,693 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.