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Identification of altered protein abundances in cholesteatoma matrix via mass spectrometry-based proteomic analysis

Overview of attention for article published in Journal of Otolaryngology - Head & Neck Surgery, November 2015
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Title
Identification of altered protein abundances in cholesteatoma matrix via mass spectrometry-based proteomic analysis
Published in
Journal of Otolaryngology - Head & Neck Surgery, November 2015
DOI 10.1186/s40463-015-0104-4
Pubmed ID
Authors

Derrick R. Randall, Phillip S. Park, Justin K. Chau

Abstract

Cholesteatoma are cyst-like structures lined with a matrix of differentiated squamous epithelium overlying connective tissue. Although epithelium normally exhibits self-limited growth, cholesteatoma matrix erodes mucosa and bone suggesting changes in matrix protein constituents that permit destructive behaviour. Differential proteomic studies can measure and compare the cholesteatoma proteome to normal tissues, revealing protein alterations that may propagate the destructive process. Human cholesteatoma matrix, cholesteatoma-involved ossicles, and normal middle ear mucosa, post-auricular skin, and non-involved ossicles were harvested. These tissues were subjected to multiplex peptide labeling followed by liquid chromatography and tandem mass spectrometry analysis. Relative protein abundances were compared and evaluated for ontologic function and putative involvement in cholesteatoma. Our methodology detected 10 764 peptides constituting 1662 unique proteins at 95 % confidence or greater. Twenty-nine candidate proteins were identified in soft tissue analysis, with 29 additional proteins showing altered abundances in bone samples. Ontologic functions and known relevance to cholesteatoma are discussed, with several candidates highlighted for their roles in epithelial integrity, evasion of apoptosis, and immunologic function. This study produced an extensive cholesteatoma proteome and identified 58 proteins with altered abundances contributing to disease pathopathysiology. As well, potential biomarkers of residual disease were highlighted. Further investigation into these proteins may provide useful options for novel therapeutics or monitoring disease status.

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The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 5%
Unknown 18 95%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 3 16%
Other 2 11%
Researcher 2 11%
Student > Bachelor 1 5%
Librarian 1 5%
Other 2 11%
Unknown 8 42%
Readers by discipline Count As %
Medicine and Dentistry 6 32%
Environmental Science 2 11%
Biochemistry, Genetics and Molecular Biology 2 11%
Agricultural and Biological Sciences 1 5%
Chemistry 1 5%
Other 1 5%
Unknown 6 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 November 2015.
All research outputs
#16,042,687
of 25,457,858 outputs
Outputs from Journal of Otolaryngology - Head & Neck Surgery
#270
of 629 outputs
Outputs of similar age
#213,757
of 393,767 outputs
Outputs of similar age from Journal of Otolaryngology - Head & Neck Surgery
#3
of 19 outputs
Altmetric has tracked 25,457,858 research outputs across all sources so far. This one is in the 36th percentile – i.e., 36% of other outputs scored the same or lower than it.
So far Altmetric has tracked 629 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,767 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.