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Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus

Overview of attention for article published in Virology Journal, December 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (70th percentile)
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

Mentioned by

twitter
1 tweeter
patent
1 patent

Citations

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38 Dimensions

Readers on

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36 Mendeley
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Title
Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus
Published in
Virology Journal, December 2015
DOI 10.1186/s12985-015-0440-z
Pubmed ID
Authors

Yang, Xiaodong Qin, Guangxiang Wang, Yuen Zhang, Youjun Shang, Zhidong Zhang

Abstract

Orf virus (ORFV) is the causative agent of Orf (also known as contagious ecthyma or contagious papular dermatitis), a severe infectious skin disease in goats, sheep and other ruminants. The rapid detection of ORFV is of great importance in disease control and highly needed. A isothermal molecular diagnostic approach, termed recombinase polymerase amplification (RPA), is considered as an novel and rapid alternative techonology to PCR assay. In the present study, a novel fluorescent probe based on RPA assay (ORFV exo RPA assay) was developed. The developed ORFV exo RPA assay was capable of as low as 100 copies of ORFV DNA /reaction and was highly specific, with no cross-reaction with closely related viruses (capripox virus, foot-and-mouth disease virus or peste des petits ruminants virus). Further assessment with clinical samples showed that the developed ORFV exo RPA assay has good correlation with qPCR assays for detection of ORFV. These results suggest that the developed ORFV exo RPA assay is suitable for rapid detection of ORFV.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 3%
Unknown 35 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 22%
Student > Master 7 19%
Student > Ph. D. Student 6 17%
Professor > Associate Professor 2 6%
Student > Bachelor 2 6%
Other 3 8%
Unknown 8 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 19%
Agricultural and Biological Sciences 6 17%
Veterinary Science and Veterinary Medicine 5 14%
Engineering 3 8%
Immunology and Microbiology 1 3%
Other 3 8%
Unknown 11 31%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 February 2019.
All research outputs
#4,447,963
of 14,860,150 outputs
Outputs from Virology Journal
#527
of 2,339 outputs
Outputs of similar age
#105,571
of 361,173 outputs
Outputs of similar age from Virology Journal
#50
of 181 outputs
Altmetric has tracked 14,860,150 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 2,339 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.4. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 361,173 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 181 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.