Title |
Revealing cellular and molecular complexity of the central nervous system using single cell sequencing
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Published in |
Stem Cell Research & Therapy, September 2018
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DOI | 10.1186/s13287-018-0985-z |
Pubmed ID | |
Authors |
Zhiwei Zeng, Nan Miao, Tao Sun |
Abstract |
The mammalian central nervous system (CNS) is one of the most complex systems, with thousands of cell types and subtypes with distinct and unique morphology and gene expression profiles. Based on classic histological methods and conventional cellular and molecular approaches, single cell sequencing is becoming a powerful tool to uncover the complexity of the CNS. In this review, we summarize the principle of single cell sequencing and highlight its use for studying the development of neural stem cells, neural progenitors, and distinct neurons. By revealing transcriptomes in each individual cell using single cell sequencing, we are now able to dissect the cellular heterogeneity of a hundred billion cells in the CNS and comprehensively investigate mechanisms of brain development and function at the cellular and molecular levels. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Austria | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 81 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 25 | 31% |
Researcher | 10 | 12% |
Unspecified | 6 | 7% |
Student > Bachelor | 5 | 6% |
Student > Postgraduate | 4 | 5% |
Other | 12 | 15% |
Unknown | 19 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 17 | 21% |
Neuroscience | 12 | 15% |
Agricultural and Biological Sciences | 9 | 11% |
Unspecified | 7 | 9% |
Medicine and Dentistry | 6 | 7% |
Other | 9 | 11% |
Unknown | 21 | 26% |