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Epigenetic effects of casein-derived opioid peptides in SH-SY5Y human neuroblastoma cells

Overview of attention for article published in Nutrition & Metabolism, December 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (94th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Mentioned by

blogs
1 blog
twitter
25 tweeters
patent
1 patent
facebook
8 Facebook pages
video
2 video uploaders

Citations

dimensions_citation
25 Dimensions

Readers on

mendeley
93 Mendeley
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Title
Epigenetic effects of casein-derived opioid peptides in SH-SY5Y human neuroblastoma cells
Published in
Nutrition & Metabolism, December 2015
DOI 10.1186/s12986-015-0050-1
Pubmed ID
Authors

Malav S. Trivedi, Nathaniel W. Hodgson, Stephen J. Walker, Geert Trooskens, Vineeth Nair, Richard C. Deth

Abstract

Casein-free, gluten-free diets have been reported to mitigate some of the inflammatory gastrointestinal and behavioral traits associated with autism, but the mechanism for this palliative effect has not been elucidated. We recently showed that the opioid peptide beta-casomorphin-7, derived from bovine (bBCM7) milk, decreases cysteine uptake, lowers levels of the antioxidant glutathione (GSH) and decreases the methyl donor S-adenosylmethionine (SAM) in both Caco-2 human GI epithelial cells and SH-SY5Y human neuroblastoma cells. While human breast milk can also release a similar peptide (hBCM-7), the bBCM7 and hBCM-7 vary greatly in potency; as the bBCM-7 is highly potent and similar to morphine in it's effects. Since SAM is required for DNA methylation, we wanted to further investigate the epigenetic effects of these food-derived opioid peptides. In the current study the main objective was to characterize functional pathways and key genes responding to DNA methylation effects of food-derived opioid peptides. SH-SY5Y neuroblastoma cells were treated with 1 μM hBCM7 and bBCM7 and RNA and DNA were isolated after 4 h with or without treatment. Transcriptional changes were assessed using a microarray approach and CpG methylation status was analyzed at 450,000 CpG sites. Functional implications from both endpoints were evaluated via Ingenuity Pathway Analysis 4.0 and KEGG pathway analysis was performed to identify biological interactions between transcripts that were significantly altered at DNA methylation or transcriptional levels (p < 0.05, FDR <0.1). Here we show that hBCM7 and bBCM7, as well as morphine, cause epigenetic changes affecting gene pathways related to gastrointestinal disease and inflammation. These epigenetic consequences exhibited the same potency order as opiate inhibition of cysteine uptake insofar as hBCM7 was less potent than bBCM7, which was less potent than morphine. Our findings indicate that epigenetic effects of milk-derived opiate peptides may contribute to GI dysfunction and inflammation in sensitive individuals. While the current study was performed using SH-SY5Y neuronal cellular models, similar actions on other cells types might combine to cause symptoms of intolerance. These actions may provide a potential contributing mechanism for the beneficial effects of a casein-free diet in alleviating gastrointestinal symptoms in neurological conditions including autism and other conditions. Lastly, our study also contributes to the evolving awareness of a "gut-brain connection".

Twitter Demographics

The data shown below were collected from the profiles of 25 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 93 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Poland 1 1%
Denmark 1 1%
Unknown 91 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 17 18%
Student > Ph. D. Student 12 13%
Student > Master 11 12%
Student > Doctoral Student 8 9%
Researcher 8 9%
Other 22 24%
Unknown 15 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 18%
Medicine and Dentistry 12 13%
Biochemistry, Genetics and Molecular Biology 11 12%
Neuroscience 8 9%
Nursing and Health Professions 7 8%
Other 17 18%
Unknown 21 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 28. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 June 2021.
All research outputs
#1,192,266
of 22,875,477 outputs
Outputs from Nutrition & Metabolism
#178
of 950 outputs
Outputs of similar age
#22,144
of 389,107 outputs
Outputs of similar age from Nutrition & Metabolism
#3
of 27 outputs
Altmetric has tracked 22,875,477 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 950 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.5. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 389,107 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.