Title |
A genome-wide survey of DNA methylation in hexaploid wheat
|
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Published in |
Genome Biology, December 2015
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DOI | 10.1186/s13059-015-0838-3 |
Pubmed ID | |
Authors |
Laura-Jayne Gardiner, Mark Quinton-Tulloch, Lisa Olohan, Jonathan Price, Neil Hall, Anthony Hall |
Abstract |
DNA methylation is an important mechanism of epigenetic gene expression control that can be passed between generations. Here, we use sodium bisulfite treatment and targeted gene enrichment to study genome-wide methylation across the three sub-genomes of allohexaploid wheat. While the majority of methylation is conserved across all three genomes we demonstrate that differential methylation exists between the sub-genomes in approximately equal proportions. We correlate sub-genome-specific promoter methylation with decreased expression levels and show that altered growing temperature has a small effect on methylation state, identifying a small but functionally relevant set of methylated genes. Finally, we demonstrate long-term methylation maintenance using a comparison between the D sub-genome of hexaploid wheat and its progenitor Aegilops tauschii. We show that tri-genome methylation is highly conserved with the diploid wheat progenitor while sub-genome-specific methylation shows more variation. |
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Geographical breakdown
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United States | 4 | 10% |
France | 2 | 5% |
Australia | 2 | 5% |
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Brunei Darussalam | 1 | 3% |
Pakistan | 1 | 3% |
Korea, Republic of | 1 | 3% |
Other | 1 | 3% |
Unknown | 15 | 38% |
Demographic breakdown
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Members of the public | 25 | 64% |
Scientists | 13 | 33% |
Science communicators (journalists, bloggers, editors) | 1 | 3% |
Mendeley readers
Geographical breakdown
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United Kingdom | 1 | <1% |
Chile | 1 | <1% |
France | 1 | <1% |
Unknown | 140 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 46 | 32% |
Student > Ph. D. Student | 34 | 24% |
Student > Doctoral Student | 9 | 6% |
Student > Master | 8 | 6% |
Other | 7 | 5% |
Other | 16 | 11% |
Unknown | 23 | 16% |
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Biochemistry, Genetics and Molecular Biology | 20 | 14% |
Environmental Science | 2 | 1% |
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Nursing and Health Professions | 1 | <1% |
Other | 3 | 2% |
Unknown | 33 | 23% |