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Novel protein chip for the detection of antibodies against infectious bronchitis virus

Overview of attention for article published in BMC Veterinary Research, September 2018
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Title
Novel protein chip for the detection of antibodies against infectious bronchitis virus
Published in
BMC Veterinary Research, September 2018
DOI 10.1186/s12917-018-1586-x
Pubmed ID
Authors

Liping Yan, Jianhua Hu, Jing Lei, Zhiyu Shi, Qian Xiao, Zhenwei Bi, Lu Yao, Yuan Li, Yuqing Chen, An Fang, Hui Li, Suquan Song, Min Liao, Jiyong Zhou

Abstract

Infectious bronchitis (IB) caused by the IB virus (IBV) can cause acute damage to chickens around the world. Therefore, rapid diagnosis and immune status determination are critical for controlling IBV outbreaks. Enzyme-linked immunosorbent assays (ELISAs) have been widely used in the detection of IBV antibodies in the early infection and continuous infection of IB because they are more sensitive and quicker than other diagnostic methods. We have developed two indirect microarray methods to detect antibodies against IBV: a chemiluminescent immunoassay test (CIT) and a rapid diagnostic test (RDT). IBV nonstructural protein 5 (nsp5) was expressed, purified from Escherichia coli, and used to spot the initiator integrated poly(dimethylsiloxane), which can provide a near "zero" background for serological assays. Compared with the IDEXX IBV Ab Test kit, CIT and RDT have a sensitivity and specificity of at least 98.88% and 91.67%, respectively. No cross-reaction was detected with antibodies against avian influenza virus subtypes (H5, H7, and H9), Newcastle disease virus, Marek's disease virus, infectious bursal disease virus, and chicken anemia virus. The coefficients of variation of the reproducibility of the intra- and inter-assays for CIT ranged from 0.8 to 18.63%. The reproducibility of RDT was consistent with the original results. The application of the IBV nsp5 protein microarray showed that the positive rate of the CIT was 96.77%, that of the nsp5 ELISA was 91.40%, and that of the RDT was 90.32%. Furthermore, the RDT, which was visible to the naked eye, could be completed within 15 min. Our results indicated that compared with nsp5 ELISA, the CIT was more sensitive, and the RDT had similar positive rates but was faster. Furthermore, the two proposed methods were specific and stable. Two microarray assays, which were rapid, specific, sensitive, and relatively simple, were developed for the detection of an antibody against IBV. These methods can be of great value for the surveillance of pathogens and monitoring the efficiency of vaccination.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 37 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 30%
Student > Ph. D. Student 6 16%
Student > Master 4 11%
Student > Bachelor 3 8%
Student > Doctoral Student 2 5%
Other 3 8%
Unknown 8 22%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 7 19%
Biochemistry, Genetics and Molecular Biology 6 16%
Medicine and Dentistry 5 14%
Agricultural and Biological Sciences 4 11%
Computer Science 1 3%
Other 3 8%
Unknown 11 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2018.
All research outputs
#20,533,782
of 23,103,903 outputs
Outputs from BMC Veterinary Research
#2,447
of 3,084 outputs
Outputs of similar age
#297,102
of 341,518 outputs
Outputs of similar age from BMC Veterinary Research
#61
of 71 outputs
Altmetric has tracked 23,103,903 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,084 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 71 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.