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Bacterial contamination of human skin allografts and antimicrobial resistance: a skin bank problem

Overview of attention for article published in BMC Microbiology, September 2018
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Title
Bacterial contamination of human skin allografts and antimicrobial resistance: a skin bank problem
Published in
BMC Microbiology, September 2018
DOI 10.1186/s12866-018-1261-1
Pubmed ID
Authors

Karine Lena Meneghetti, Micaela do Canto Canabarro, Letícia Muner Otton, Thaís dos Santos Hain, Mercedes Passos Geimba, Gertrudes Corção

Abstract

Bacterial contamination remains the major problem in skin banks, even after antimicrobial treatment, and results in high rates of tissue discarding. This study aimed to analyze bacterial contamination in 32 human skin allografts from the skin bank of Dr. Roberto Corrêa Chem from the Hospital Complex Santa Casa de Misericórdia de Porto Alegre. These samples were already discarded due to microbial contamination. The identification of the bacteria isolated from skin allografts was performed by matrix assisted laser desorption ionization-time of flight. The antimicrobial susceptibility of the isolates to six different classes of antimicrobials was determined using the disk-diffusion agar method, and the evaluation of the inhibitory potential was determined by the minimal inhibitory concentration (50/90) of antimicrobials already used in the skin bank and those that most isolates were susceptible to. A total of 21 (65.6%) skin samples were contaminated with Gram-positive bacteria: 1 (4.7%) with Paenibacillus sp., 12 (61.9%) with Bacillus sp., 6 (28.5%) with Staphylococcus sp., and 2 (9.5%) with Bacillus sp. and Staphylococcus sp. Several resistance profiles, including multiresistance, were found among the isolates. Most of the isolates were susceptible to at least one of the antimicrobials used in the skin bank. All isolates were susceptible to amikacin, gentamicin, and tetracycline, which demonstrated the best inhibitory activities against the isolates and were considered as potential candidates for new antimicrobial treatments. Bacillus, Paenibacillus, and Staphylococcus were isolated from the skin allografts, thus demonstrating the predominance of Gram-positive bacteria contamination. Other factors not related to the resistance phenotype may also be involved in the persistence of bacterial isolates in the skin allografts after antibiotic treatment. Gentamicin, amikacin, and tetracycline can be considered as an option for a more effective treatment cocktail.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 42 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 19%
Student > Master 6 14%
Student > Doctoral Student 3 7%
Researcher 3 7%
Other 2 5%
Other 4 10%
Unknown 16 38%
Readers by discipline Count As %
Medicine and Dentistry 9 21%
Biochemistry, Genetics and Molecular Biology 6 14%
Immunology and Microbiology 5 12%
Agricultural and Biological Sciences 1 2%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Other 2 5%
Unknown 18 43%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 September 2018.
All research outputs
#12,019,702
of 13,555,083 outputs
Outputs from BMC Microbiology
#1,644
of 2,033 outputs
Outputs of similar age
#228,338
of 264,839 outputs
Outputs of similar age from BMC Microbiology
#21
of 24 outputs
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So far Altmetric has tracked 2,033 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.