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Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors

Overview of attention for article published in Journal for Immunotherapy of Cancer, December 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (71st percentile)
  • Good Attention Score compared to outputs of the same age and source (70th percentile)

Mentioned by

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2 tweeters
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2 patents

Citations

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49 Dimensions

Readers on

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74 Mendeley
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Title
Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors
Published in
Journal for Immunotherapy of Cancer, December 2015
DOI 10.1186/s40425-015-0099-4
Pubmed ID
Authors

Joanne A. Hammill, Heather VanSeggelen, Christopher W. Helsen, Galina F. Denisova, Carole Evelegh, Daniela G. M. Tantalo, Jennifer D. Bassett, Jonathan L. Bramson

Abstract

Adoptive cell transfer of tumor-specific T lymphocytes (T cells) is proving to be an effective strategy for treating established tumors in cancer patients. One method of generating these cells is accomplished through engineering bulk T cell populations to express chimeric antigen receptors (CARs), which are specific for tumor antigens. Traditionally, these CARs are targeted against tumor antigens using single-chain antibodies (scFv). Here we describe the use of a designed ankyrin repeat protein (DARPin) as the tumor-antigen targeting domain. We prepared second generation anti-HER2 CARs that were targeted to the tumor antigen by either a DARPin or scFv. The CARs were engineered into human and murine T cells. We then compared the ability of CARs to trigger cytokine production, degranulation and cytotoxicity. The DARPin CARs displayed reduced surface expression relative to scFv CARs in murine cells but both CARs were expressed equally well on human T cells, suggesting that there may be a processing issue with the murine variants. In both the murine and human systems, the DARPin CARs were found to be highly functional, triggering cytokine and cytotoxic responses that were similar to those triggered by the scFv CARs. These findings demonstrate the utility of DARPins as CAR-targeting agents and open up an avenue for the generation of CARs with novel antigen binding attributes.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 74 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 19 26%
Researcher 15 20%
Student > Ph. D. Student 12 16%
Student > Master 7 9%
Student > Doctoral Student 5 7%
Other 11 15%
Unknown 5 7%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 24 32%
Agricultural and Biological Sciences 19 26%
Medicine and Dentistry 8 11%
Immunology and Microbiology 7 9%
Engineering 3 4%
Other 7 9%
Unknown 6 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 March 2019.
All research outputs
#5,107,997
of 17,358,590 outputs
Outputs from Journal for Immunotherapy of Cancer
#869
of 1,743 outputs
Outputs of similar age
#103,876
of 373,412 outputs
Outputs of similar age from Journal for Immunotherapy of Cancer
#32
of 107 outputs
Altmetric has tracked 17,358,590 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 1,743 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 13.7. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 373,412 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 107 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.