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Organ culture of seminiferous tubules using a modified soft agar culture system

Overview of attention for article published in Stem Cell Research & Therapy, September 2018
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Title
Organ culture of seminiferous tubules using a modified soft agar culture system
Published in
Stem Cell Research & Therapy, September 2018
DOI 10.1186/s13287-018-0997-8
Pubmed ID
Authors

Keykavos Gholami, Gholamreza Pourmand, Morteza Koruji, Sepideh Ashouri, Mehdi Abbasi

Abstract

In-vitro spermatogenesis in mammalian species is considered an important topic in reproductive biology. New strategies for achieving a complete version of spermatogenesis ex vivo have been conducted using an organ culture method or culture of testicular cells in a three-dimensional soft agar culture system (SACS). The aim of this study was to develop a new method that supports spermatogenesis to the meiotic phase and morphologically mature spermatozoa through the culture of testicular cells and seminiferous tubules (STs) in a modified SACS, respectively. First, enzymatically dissociated testicular cells and mechanically dissociated STs of neonatal mice were separately embedded in agarose and then placed on the flat surface of agarose gel half-soaked in the medium to continue culture with a gas-liquid interphase method. Following 40 days of culture, the meiotic (Scp3) and post-meiotic (Acr) gene expression in aggregates and STs was confirmed by real-time polymerase chain reaction. These results were complemented by immunohistochemistry. The presence of morphologically mature spermatozoa in the frozen sections of STs was demonstrated with hematoxylin and eosin staining. We observed Plzf- or Integrin α6-positive spermatogonia in both cultures after 40 days, indicating the potency of the culture system for both self-renewal and differentiation. This technique can be used as a valuable approach for performing research on spermatogenesis and translating it into the human clinical setting.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 37 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 19%
Researcher 6 16%
Student > Master 5 14%
Student > Doctoral Student 4 11%
Student > Bachelor 2 5%
Other 3 8%
Unknown 10 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 24%
Medicine and Dentistry 4 11%
Engineering 4 11%
Veterinary Science and Veterinary Medicine 2 5%
Agricultural and Biological Sciences 2 5%
Other 3 8%
Unknown 13 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2018.
All research outputs
#20,535,139
of 23,105,443 outputs
Outputs from Stem Cell Research & Therapy
#2,073
of 2,439 outputs
Outputs of similar age
#296,864
of 341,556 outputs
Outputs of similar age from Stem Cell Research & Therapy
#50
of 64 outputs
Altmetric has tracked 23,105,443 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,439 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.