Title |
Treatment with G-CSF reduces acute myeloid leukemia blast viability in the presence of bone marrow stroma
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Published in |
Cancer Cell International, December 2015
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DOI | 10.1186/s12935-015-0272-3 |
Pubmed ID | |
Authors |
Meritxell Nomdedeu, María Carmen Lara-Castillo, Amaia Etxabe, Josep María Cornet-Masana, Marta Pratcorona, Marina Díaz-Beyá, Xavier Calvo, María Rozman, Dolors Costa, Jordi Esteve, Ruth M. Risueño |
Abstract |
The resulting clinical impact of the combined use of G-CSF with chemotherapy as a chemosensitizing strategy for treatment of acute myeloid leukemia (AML) patients is still controversial. In this study, the effect of ex vivo treatment with G-CSF on AML primary blasts was studied. Peripheral blood mononuclear cells from AML patients were treated with G-CSF at increasing doses, alone or in co-culture with HS-5 stromal cells. Cell viability and surface phenotype was determined by flow cytometry 72 h after treatment. For clonogenicity assays, AML primary samples were treated for 18 h with G-CSF at increasing concentrations and cultured in methyl-cellulose for 14 days. Colonies were counted based on cellularity and morphology criteria. The presence of G-CSF reduced the overall viability of AML cells co-cultured with bone marrow stroma; whereas, in absence of stroma, a negligible effect was observed. Moreover, clonogenic capacity of AML cells was significantly reduced upon treatment with G-CSF. Interestingly, reduction in the AML clonogenic capacity correlated with the sensitivity to chemotherapy observed in vivo. These ex vivo results would provide a biological basis to data available from studies showing a clinical benefit with the use of G-CSF as a priming agent in patients with a chemosensitive AML and would support implementation of further studies exploring new strategies of chemotherapy priming in AML. |
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Mendeley readers
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Researcher | 7 | 24% |
Professor > Associate Professor | 3 | 10% |
Student > Master | 3 | 10% |
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Other | 3 | 10% |
Unknown | 3 | 10% |
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Psychology | 1 | 3% |
Other | 1 | 3% |
Unknown | 6 | 21% |