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Vemurafenib-resistant BRAF selects alternative branch points different from its wild-type BRAF in intron 8 for RNA splicing

Overview of attention for article published in Cell & Bioscience, December 2015
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Mentioned by

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2 tweeters

Citations

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4 Dimensions

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16 Mendeley
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Title
Vemurafenib-resistant BRAF selects alternative branch points different from its wild-type BRAF in intron 8 for RNA splicing
Published in
Cell & Bioscience, December 2015
DOI 10.1186/s13578-015-0061-7
Pubmed ID
Authors

Masahiko Ajiro, Zhi-Ming Zheng

Abstract

One mechanism of resistance of the melanoma-associated BRAF kinase to its small molecule inhibitor vemurafenib is by point mutations in its intron 8 resulting in exons 4-8 skipping. In this report, we carried out in vitro BRAF RNA splicing assays and lariat RT-PCR to map the intron 8 branch points in wild-type and BRAF mutants. We identify multiple branch points (BP) in intron 8 of both wild-type (wt) and vemurafenib-resistant BRAF RNA. In wt BRAF, BPs are located at -29A, -28A and -26A, whereas in a vemurafenib-resistant BRAF splicing mutant, BPs map to -22A, -18A and -15A, proximal to the intron 8 3' splice site. This finding of a distal-to-proximal shift of the branch point sequence in BRAF splicing in response to point-mutations in intron 8 provides insight into the regulation of BRAF alternative splicing upon vemurafenib resistance.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 19%
Researcher 3 19%
Student > Ph. D. Student 3 19%
Student > Doctoral Student 2 13%
Professor 1 6%
Other 2 13%
Unknown 2 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 25%
Agricultural and Biological Sciences 4 25%
Social Sciences 2 13%
Medicine and Dentistry 2 13%
Chemistry 2 13%
Other 0 0%
Unknown 2 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 December 2015.
All research outputs
#10,319,904
of 16,169,362 outputs
Outputs from Cell & Bioscience
#155
of 463 outputs
Outputs of similar age
#198,277
of 371,119 outputs
Outputs of similar age from Cell & Bioscience
#24
of 56 outputs
Altmetric has tracked 16,169,362 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 463 research outputs from this source. They receive a mean Attention Score of 2.2. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 371,119 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 56 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.