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Attention Score in Context
| Title |
Effects of SNPs and alternative splicing within HGF gene on its expression patterns in Qinchuan cattle
|
|---|---|
| Published in |
Journal of Animal Science and Biotechnology, December 2015
|
| DOI | 10.1186/s40104-015-0059-3 |
| Pubmed ID | |
| Authors |
Hanfang Cai, Yang Zhou, Wenchao Jia, Bowen Zhang, Xianyong Lan, Chuzhao Lei, Xintang Fang, Hong Chen |
| Abstract |
Identification of genetic variants, including SNPs (Single Nucleotide Polymorphisms), CNVs (Copy Number Variations) and alternative splicing, within functional genes has received increasing attention in animal science research. HGF (Hepatocyte Growth Factor) is a very important growth factor that works as a mitogen or a morphogen during tissue growth, development and regeneration. However, to date, the functions of genetic variants within the bovine HGF gene, particularly their effects on mRNA expression, have not been determined well. The present study aimed to perform association analysis between genetic variants and mRNA expression for the bovine HGF gene in Qinchuan cattle using various strategies, including PCR-RFLP (Restriction Fragment Length Polymorphism), qPCR (Quantitative Real-time quantitative PCR), TA cloning, DNA sequencing and bioinformatics analysis. A total of five SNPs were identified and only SV1 locus significantly affected HGF mRNA expression in fetal skeletal muscle (P < 0.05). Heterozygous genotype individuals showed significantly higher HGF expression (P < 0.05), which was significantly greater in the "CTCCAGGGTT" combined genotype than that in the "CCCCGGGGTT" combined genotype (P < 0.05). In addition, two alternative splicing variations, HGF-W and HGF-M, were identified, which resulted from alternative 3' splice sites of exon 5, and HGF-W showed higher mRNA levels than HGF-M in all tissues. In summary, genetic variations within the HGF gene affected mRNA expression. These findings provide new insight into the molecular characteristics and functions of bovine HGF. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 29% |
| Student > Doctoral Student | 2 | 14% |
| Student > Bachelor | 2 | 14% |
| Other | 1 | 7% |
| Student > Master | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 4 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 21% |
| Biochemistry, Genetics and Molecular Biology | 2 | 14% |
| Veterinary Science and Veterinary Medicine | 1 | 7% |
| Physics and Astronomy | 1 | 7% |
| Medicine and Dentistry | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 6 | 43% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 December 2015.
All research outputs
#17,286,645
of 25,374,917 outputs
Outputs from Journal of Animal Science and Biotechnology
#455
of 903 outputs
Outputs of similar age
#240,170
of 396,426 outputs
Outputs of similar age from Journal of Animal Science and Biotechnology
#4
of 8 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 903 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
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We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.