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Simultaneous detection of eight immunosuppressive chicken viruses using a GeXP analyser-based multiplex PCR assay

Overview of attention for article published in Virology Journal, December 2015
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Title
Simultaneous detection of eight immunosuppressive chicken viruses using a GeXP analyser-based multiplex PCR assay
Published in
Virology Journal, December 2015
DOI 10.1186/s12985-015-0455-5
Pubmed ID
Authors

Tingting Zeng, Zhixun Xie, Liji Xie, Xianwen Deng, Zhiqin Xie, Sisi Luo, Li Huang, Jiaoling Huang

Abstract

Immunosuppressive viruses are frequently found as co-infections in the chicken industry, potentially causing serious economic losses. Because traditional molecular biology methods have limited detection ability, a rapid, high-throughput method for the differential diagnosis of these viruses is needed. The objective of this study is to develop a GenomeLab Gene Expression Profiler Analyser-based multiplex PCR method (GeXP-multiplex PCR) for simultaneous detection of eight immunosuppressive chicken viruses. Using chimeric primers, eight such viruses, including Marek's disease virus (MDV), three subgroups of avian leucosis virus (ALV-A/B/J), reticuloendotheliosis virus (REV), infectious bursal disease virus (IBDV), chicken infectious anaemia virus (CIAV) and avian reovirus (ARV), were amplified and identified by their respective amplicon sizes. The specificity and sensitivity of the optimised GeXP-multiplex PCR assay were evaluated, and the data demonstrated that this technique could selectively amplify these eight viruses at a sensitivity of 100 copies/20 μl when all eight viruses were present. Among 300 examined clinical specimens, 190 were found to be positive for immunosuppressive viruses according to this novel assay. The GeXP-multiplex PCR assay is a high-throughput, sensitive and specific method for the detection of eight immunosuppressive viruses and can be used for differential diagnosis and molecular epidemiological surveys.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 31%
Researcher 7 27%
Student > Bachelor 3 12%
Student > Ph. D. Student 2 8%
Student > Doctoral Student 1 4%
Other 3 12%
Unknown 2 8%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 7 27%
Agricultural and Biological Sciences 6 23%
Immunology and Microbiology 3 12%
Engineering 2 8%
Computer Science 2 8%
Other 2 8%
Unknown 4 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2015.
All research outputs
#5,138,588
of 6,892,495 outputs
Outputs from Virology Journal
#1,168
of 1,495 outputs
Outputs of similar age
#205,124
of 300,538 outputs
Outputs of similar age from Virology Journal
#43
of 51 outputs
Altmetric has tracked 6,892,495 research outputs across all sources so far. This one is in the 14th percentile – i.e., 14% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,495 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
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We're also able to compare this research output to 51 others from the same source and published within six weeks on either side of this one. This one is in the 3rd percentile – i.e., 3% of its contemporaries scored the same or lower than it.