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Adipose-derived endothelial and mesenchymal stem cells enhance vascular network formation on three-dimensional constructs in vitro

Overview of attention for article published in Stem Cell Research & Therapy, January 2016
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Title
Adipose-derived endothelial and mesenchymal stem cells enhance vascular network formation on three-dimensional constructs in vitro
Published in
Stem Cell Research & Therapy, January 2016
DOI 10.1186/s13287-015-0251-6
Pubmed ID
Authors

Alina Freiman, Yulia Shandalov, Dekel Rozenfeld, Erez Shor, Sofia Segal, Dror Ben-David, Shai Meretzki, Dana Egozi, Shulamit Levenberg

Abstract

Adipose-derived mesenchymal stem cells (MSCs) have been gaining fame mainly due to their vast clinical potential, simple isolation methods and minimal donor site morbidity. Adipose-derived MSCs and microvascular endothelial cells have been shown to bear angiogenic and vasculogenic capabilities. We hypothesized that co-culture of human adipose-derived MSCs with human adipose-derived microvascular endothelial cells (HAMECs) will serve as an effective cell pair to induce angiogenesis and vessel-like network formation in three-dimensional scaffolds in vitro. HAMECs or human umbilical vein endothelial cells (HUVECs) were co-cultured on scaffolds with either MSCs or human neonatal dermal fibroblasts. Cells were immunofluorescently stained within the scaffolds at different time points post-seeding. Various analyses were performed to determine vessel length, complexity and degree of maturity. The HAMEC:MSC combination yielded the most organized and complex vascular elements within scaffolds, and in the shortest period of time, when compared to the other tested cell combinations. These differences were manifested by higher network complexity, more tube alignment and higher α-smooth muscle actin expression. Moreover, these generated microvessels further matured and developed during the 14-day incubation period within the three-dimensional microenvironment. These data demonstrate optimal vascular network formation upon co-culture of microvascular endothelial cells and adipose-derived MSCs in vitro and constitute a significant step in appreciation of the potential of microvascular endothelial cells and MSCs in different tissue engineering applications that can also be advantageous in in vivo studies.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 168 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 2 1%
United Kingdom 1 <1%
Japan 1 <1%
Unknown 164 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 45 27%
Student > Master 26 15%
Researcher 20 12%
Student > Bachelor 19 11%
Student > Doctoral Student 13 8%
Other 21 13%
Unknown 24 14%
Readers by discipline Count As %
Engineering 34 20%
Biochemistry, Genetics and Molecular Biology 29 17%
Medicine and Dentistry 29 17%
Agricultural and Biological Sciences 23 14%
Materials Science 8 5%
Other 11 7%
Unknown 34 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2016.
All research outputs
#15,353,264
of 22,837,982 outputs
Outputs from Stem Cell Research & Therapy
#1,343
of 2,420 outputs
Outputs of similar age
#231,684
of 394,936 outputs
Outputs of similar age from Stem Cell Research & Therapy
#33
of 43 outputs
Altmetric has tracked 22,837,982 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,420 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 394,936 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.