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CD146/MCAM defines functionality of human bone marrow stromal stem cell populations

Overview of attention for article published in Stem Cell Research & Therapy, January 2016
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • Good Attention Score compared to outputs of the same age and source (72nd percentile)

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1 blog
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2 X users

Citations

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77 Dimensions

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108 Mendeley
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Title
CD146/MCAM defines functionality of human bone marrow stromal stem cell populations
Published in
Stem Cell Research & Therapy, January 2016
DOI 10.1186/s13287-015-0266-z
Pubmed ID
Authors

Linda Harkness, Walid Zaher, Nicholas Ditzel, Adiba Isa, Moustapha Kassem

Abstract

Identification of surface markers for prospective isolation of functionally homogenous populations of human skeletal (stromal, mesenchymal) stem cells (hMSCs) is highly relevant for cell therapy protocols. Thus, we examined the possible use of CD146 to subtype a heterogeneous hMSC population. Using flow cytometry and cell sorting, we isolated two distinct hMSC-CD146(+) and hMSC-CD146(-) cell populations from the telomerized human bone marrow-derived stromal cell line (hMSC-TERT). Cells were examined for differences in their size, shape and texture by using high-content analysis and additionally for their ability to differentiate toward osteogenesis in vitro and form bone in vivo, and their migrational ability in vivo and in vitro was investigated. In vitro, the two cell populations exhibited similar growth rate and differentiation capacity to osteoblasts and adipocytes on the basis of gene expression and protein production of lineage-specific markers. In vivo, hMSC-CD146(+) and hMSC-CD146(-) cells formed bone and bone marrow organ when implanted subcutaneously in immune-deficient mice. Bone was enriched in hMSC-CD146(-) cells (12.6 % versus 8.1 %) and bone marrow elements enriched in implants containing hMSC-CD146(+) cells (0.5 % versus 0.05 %). hMSC-CD146(+) cells exhibited greater chemotactic attraction in a transwell migration assay and, when injected intravenously into immune-deficient mice following closed femoral fracture, exhibited wider tissue distribution and significantly increased migration ability as demonstrated by bioluminescence imaging. Our studies demonstrate that CD146 defines a subpopulation of hMSCs capable of bone formation and in vivo trans-endothelial migration and thus represents a population of hMSCs suitable for use in clinical protocols of bone tissue regeneration.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 <1%
Australia 1 <1%
Brazil 1 <1%
Unknown 105 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 28 26%
Researcher 19 18%
Student > Master 10 9%
Student > Doctoral Student 8 7%
Student > Bachelor 7 6%
Other 17 16%
Unknown 19 18%
Readers by discipline Count As %
Medicine and Dentistry 25 23%
Agricultural and Biological Sciences 21 19%
Biochemistry, Genetics and Molecular Biology 19 18%
Engineering 6 6%
Immunology and Microbiology 5 5%
Other 8 7%
Unknown 24 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 January 2016.
All research outputs
#3,092,368
of 22,840,638 outputs
Outputs from Stem Cell Research & Therapy
#259
of 2,420 outputs
Outputs of similar age
#56,598
of 394,936 outputs
Outputs of similar age from Stem Cell Research & Therapy
#11
of 43 outputs
Altmetric has tracked 22,840,638 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,420 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 394,936 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.