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Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis

Overview of attention for article published in Arthritis Research & Therapy, April 2015
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Title
Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis
Published in
Arthritis Research & Therapy, April 2015
DOI 10.1186/s13075-015-0619-0
Pubmed ID
Authors

Ming Zong, Tianbao Lu, Shasha Fan, Hui Zhang, Ruhan Gong, Lishan Sun, Zhiyan Fu, Lieying Fan

Abstract

Fibroblast-like synoviocytes (FLS) play an important role in the pathogenesis of rheumatoid arthritis (RA). This study aimed to investigate the role of glucose 6-phosphate isomerase (GPI) in the proliferation of RA-FLS. The distribution of GPI in synovial tissues from RA and osteoarthritis (OA) patients was examined by immunohistochemical analysis. FLS were isolated and cultured, cellular GPI level was detected by real-time polymerase chain reaction (PCR) and Western blot analysis, and secreted GPI was detected by Western blot and enzyme-linked immunosorbent assay (ELISA). Adriamycin (ADR) was used to induce apoptosis. Cell proliferation was determined by MTS assay. Flow cytometry was used to detect cell cycle and apoptosis. Secreted proinflammatory cytokines were measured by ELISA. GPI was abundant in RA-FLS and was an autocrine factor of FLS. The proliferation of both RA and OA FLS was increased after GPI overexpression but was decreased after GPI knockdown. Meanwhile, exogenous GPI stimulated while GPI antibody inhibited FLS proliferation. GPI positively regulated its receptor glycoprotein 78 (gp78) and promoted G1/S phase transition via extracellular regulated protein kinases (ERK) activation and Cyclin D1 upregulation. GPI inhibited ADR-induced apoptosis accompanied by decreased Fas and increased survivin in RA FLS. Furthermore, GPI increased the secretion of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) by FLS. GPI plays pathophysiologic role in RA by stimulating the proliferation, inhibiting the apoptosis and increasing proinflammatory cytokine secretion of FLS.

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Mendeley readers

The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 2%
Unknown 52 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 17%
Student > Master 8 15%
Researcher 7 13%
Student > Postgraduate 6 11%
Student > Doctoral Student 5 9%
Other 9 17%
Unknown 9 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 28%
Medicine and Dentistry 10 19%
Agricultural and Biological Sciences 8 15%
Immunology and Microbiology 5 9%
Nursing and Health Professions 1 2%
Other 1 2%
Unknown 13 25%