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Alternative cleavage and polyadenylation in spermatogenesis connects chromatin regulation with post-transcriptional control

Overview of attention for article published in BMC Biology, January 2016
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (76th percentile)

Mentioned by

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9 tweeters
reddit
1 Redditor

Readers on

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74 Mendeley
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Title
Alternative cleavage and polyadenylation in spermatogenesis connects chromatin regulation with post-transcriptional control
Published in
BMC Biology, January 2016
DOI 10.1186/s12915-016-0229-6
Pubmed ID
Authors

Wencheng Li, Ji Yeon Park, Dinghai Zheng, Mainul Hoque, Ghassan Yehia, Bin Tian

Abstract

Most mammalian genes display alternative cleavage and polyadenylation (APA). Previous studies have indicated preferential expression of APA isoforms with short 3' untranslated regions (3'UTRs) in testes. By deep sequencing of the 3' end region of poly(A) + transcripts, we report widespread shortening of 3'UTR through APA during the first wave of spermatogenesis in mouse, with 3'UTR size being the shortest in spermatids. Using genes without APA as a control, we show that shortening of 3'UTR eliminates destabilizing elements, such as U-rich elements and transposable elements, which appear highly potent during spermatogenesis. We additionally found widespread regulation of APA events in introns and exons that can affect the coding sequence of transcripts and global activation of antisense transcripts upstream of the transcription start site, suggesting modulation of splicing and initiation of transcription during spermatogenesis. Importantly, genes that display significant 3'UTR shortening tend to have functions critical for further sperm maturation, and testis-specific genes display greater 3'UTR shortening than ubiquitously expressed ones, indicating functional relevance of APA to spermatogenesis. Interestingly, genes with shortened 3'UTRs tend to have higher RNA polymerase II and H3K4me3 levels in spermatids as compared to spermatocytes, features previously known to be associated with open chromatin state. Our data suggest that open chromatin may create a favorable cis environment for 3' end processing, leading to global shortening of 3'UTR during spermatogenesis. mRNAs with shortened 3'UTRs are relatively stable thanks to evasion of powerful mRNA degradation mechanisms acting on 3'UTR elements. Stable mRNAs generated in spermatids may be important for protein production at later stages of sperm maturation, when transcription is globally halted.

Twitter Demographics

The data shown below were collected from the profiles of 9 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Italy 1 1%
Canada 1 1%
Unknown 72 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 22 30%
Researcher 15 20%
Student > Master 10 14%
Student > Postgraduate 5 7%
Professor > Associate Professor 4 5%
Other 10 14%
Unknown 8 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 41%
Biochemistry, Genetics and Molecular Biology 22 30%
Nursing and Health Professions 2 3%
Veterinary Science and Veterinary Medicine 1 1%
Unspecified 1 1%
Other 7 9%
Unknown 11 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 November 2018.
All research outputs
#3,429,232
of 13,777,184 outputs
Outputs from BMC Biology
#717
of 1,204 outputs
Outputs of similar age
#79,913
of 337,259 outputs
Outputs of similar age from BMC Biology
#1
of 1 outputs
Altmetric has tracked 13,777,184 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 1,204 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.8. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 337,259 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them