Title |
Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure
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Published in |
Biotechnology for Biofuels and Bioproducts, January 2016
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DOI | 10.1186/s13068-016-0424-2 |
Pubmed ID | |
Authors |
Shuhei Ota, Kenshiro Oshima, Tomokazu Yamazaki, Sangwan Kim, Zhe Yu, Mai Yoshihara, Kohei Takeda, Tsuyoshi Takeshita, Aiko Hirata, Kateřina Bišová, Vilém Zachleder, Masahira Hattori, Shigeyuki Kawano |
Abstract |
Algae have attracted attention as sustainable producers of lipid-containing biomass for food, animal feed, and for biofuels. Parachlorella kessleri, a unicellular green alga belonging to the class Trebouxiophyceae, achieves very high biomass, lipid, and starch productivity levels. However, further biotechnological exploitation has been hampered by a lack of genomic information. Here, we sequenced the whole genome and transcriptome, and analyzed the behavior of P. kessleri NIES-2152 under lipid production-inducing conditions. The assembly includes 13,057 protein-coding genes in a 62.5-Mbp nuclear genome. Under conditions of sulfur deprivation, lipid accumulation was correlated with the transcriptomic induction of enzymes involved in sulfur metabolism, triacylglycerol (TAG) synthesis, autophagy, and remodeling of light-harvesting complexes. Three-dimensional transmission electron microscopy (3D-TEM) revealed extensive alterations in cellular anatomy accompanying lipid hyperaccumulation. The present 3D-TEM results, together with transcriptomic data support the finding that upregulation of TAG synthesis and autophagy are potential key mediators of the hyperaccumulation of lipids under conditions of nutrient stress. |
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Geographical breakdown
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Germany | 1 | 20% |
Unknown | 4 | 80% |
Demographic breakdown
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Members of the public | 4 | 80% |
Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
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France | 1 | <1% |
Brazil | 1 | <1% |
United Kingdom | 1 | <1% |
Mexico | 1 | <1% |
Denmark | 1 | <1% |
Unknown | 123 | 96% |
Demographic breakdown
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Student > Ph. D. Student | 28 | 22% |
Researcher | 24 | 19% |
Student > Bachelor | 18 | 14% |
Student > Master | 12 | 9% |
Professor > Associate Professor | 7 | 5% |
Other | 16 | 13% |
Unknown | 23 | 18% |
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Biochemistry, Genetics and Molecular Biology | 24 | 19% |
Chemical Engineering | 6 | 5% |
Environmental Science | 6 | 5% |
Engineering | 5 | 4% |
Other | 10 | 8% |
Unknown | 32 | 25% |