Title |
EV71 virus-like particles produced by co-expression of capsid proteins in yeast cells elicit humoral protective response against EV71 lethal challenge
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Published in |
BMC Research Notes, January 2016
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DOI | 10.1186/s13104-015-1780-x |
Pubmed ID | |
Authors |
Xiaowen Wang, Xiangqian Xiao, Miao Zhao, Wei Liu, Lin Pang, Xin Sun, Shan Cen, Burton B. Yang, Yuming Huang, Wang Sheng, Yi Zeng |
Abstract |
Enterovirus 71 (EV71) is the most common causative pathogens of hand, foot and mouth disease (HFMD) associated with severe neurological complications. There is a great need to develop prophylactic vaccine against EV71 infection. EV71 virus-like particle (VLP) was produced in yeast expression system by the co-expression of four EV71 structural proteins VP1-VP4. Immunization with the recombinant VLPs elicited potent anti-EV71 antibody responses in adult mice and anti-VLP sera were able to neutralize EV71 virus in vitro. Neonatal mice model demonstrated VLP immunization conferred protection to suckling mice against the lethal viral challenge. Co-expression of four EV71 structural proteins VP1-VP4 in yeast expression systems is an effective method to produce EV71 VLPs. VLP-based vaccine shows great potential to prevent EV71 infection. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 31 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 5 | 16% |
Student > Ph. D. Student | 4 | 13% |
Student > Master | 3 | 10% |
Student > Postgraduate | 3 | 10% |
Other | 2 | 6% |
Other | 5 | 16% |
Unknown | 9 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 23% |
Biochemistry, Genetics and Molecular Biology | 5 | 16% |
Immunology and Microbiology | 4 | 13% |
Medicine and Dentistry | 2 | 6% |
Nursing and Health Professions | 1 | 3% |
Other | 3 | 10% |
Unknown | 9 | 29% |