↓ Skip to main content

Human hepatic stem cells transplanted into a fulminant hepatic failure Alb-TRECK/SCID mouse model exhibit liver reconstitution and drug metabolism capabilities

Overview of attention for article published in Stem Cell Research & Therapy, March 2015
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
21 Dimensions

Readers on

mendeley
36 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Human hepatic stem cells transplanted into a fulminant hepatic failure Alb-TRECK/SCID mouse model exhibit liver reconstitution and drug metabolism capabilities
Published in
Stem Cell Research & Therapy, March 2015
DOI 10.1186/s13287-015-0038-9
Pubmed ID
Authors

Ran-Ran Zhang, Yun-Wen Zheng, Bin Li, Tomonori Tsuchida, Yasuharu Ueno, Yun-Zhong Nie, Hideki Taniguchi

Abstract

Chimeric mice with humanized livers were recently established by transplanting human hepatocytes. This mouse model that is repopulated with functional human hepatocytes could be a useful tool for investigating human hepatic cell biology and drug metabolism and for other preclinical applications. Successfully transplanting human hepatocytes into mice requires that recipient mice with liver failure do not reject these human cells and provide a suitable microenvironment (supportive niche) to promote human donor cell expansion and differentiation. To overcome the limitations of current mouse models, we used Alb-TRECK/SCID (TRECK: Toxin Receptor Mediated Cell Knockout) mice for in vivo human immature hepatocyte differentiation and humanized liver generation. 1.5 μg/kg diphtheria toxin (DT) was administrated into 8-week-old Alb-TRECK/SCID mice, and the degree of liver damage was assessed by serum aspartate aminotransferase (AST) activity levels. Forty-eight hours later, mice livers were sampled for histological analyses, and the human donor cells were then transplanted into mice livers on the same day. Chimeric rate and survival rate after cell transplantation was evaluated. Expressions of human hepatic related genes were detected. A human albumin ELISA was done after 50 days of transplantation. On day 60 after transplantation, drug metabolism was examined in mice. Both human primary fetal liver cells (FLCs) and hepatic stem cells (HpSCs) were successfully repopulated in the livers of Alb-TRECK/SCID mice that developed lethal fulminant hepatic failure after administering DT; the repopulation rate in some mice was nearly 100%. Compared with human primary FLCs, human HpSCs transplantation rescued Alb-TRECK/SCID mice with lethal fulminant hepatic failure, and human HpSCs-derived humanized livers secreted more human albumin (ALB) into mouse sera and also functioned as a "human liver" that could metabolize the drugs ketoprofen and debrisoquine. Our model of a humanized liver in Alb-TRECK/SCID mice may provide for functional applications such as drug metabolism, drug to drug interactions, and promote other in vivo and in vitro studies.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 36 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 19%
Student > Bachelor 6 17%
Student > Ph. D. Student 5 14%
Student > Postgraduate 3 8%
Student > Doctoral Student 2 6%
Other 7 19%
Unknown 6 17%
Readers by discipline Count As %
Medicine and Dentistry 12 33%
Biochemistry, Genetics and Molecular Biology 8 22%
Agricultural and Biological Sciences 4 11%
Nursing and Health Professions 2 6%
Unspecified 1 3%
Other 2 6%
Unknown 7 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2016.
All research outputs
#20,303,950
of 22,842,950 outputs
Outputs from Stem Cell Research & Therapy
#2,048
of 2,420 outputs
Outputs of similar age
#223,014
of 263,409 outputs
Outputs of similar age from Stem Cell Research & Therapy
#61
of 66 outputs
Altmetric has tracked 22,842,950 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,420 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,409 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 66 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.