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Colorectal cancer-derived microvesicles modulate differentiation of human monocytes to macrophages

Overview of attention for article published in Journal of Translational Medicine, February 2016
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Title
Colorectal cancer-derived microvesicles modulate differentiation of human monocytes to macrophages
Published in
Journal of Translational Medicine, February 2016
DOI 10.1186/s12967-016-0789-9
Pubmed ID
Authors

Monika Baj-Krzyworzeka, Bożenna Mytar, Rafał Szatanek, Marcin Surmiak, Kazimierz Węglarczyk, Jarek Baran, Maciej Siedlar

Abstract

Tumour-derived microvesicles (TMVs) are important players in tumour progression, modulating biological activity of immune cells e.g. lymphocytes, monocytes and macrophages. This phenomenon is particularly interesting in the progression of colon cancer, as macrophages in this type of tumour are relevant for the recovery processes. In the present study, the role of colon cancer cell-derived microvesicles in monocyte differentiation and activity profile (polarization) was investigated. Monocyte-derived macrophages (MDM) were differentiated in vitro in the presence of TMVs obtained from colon cancer: Caco-2, SW620, LoVo or SW480 cell lines and analysed according to their morphology and biological functions, as defined by cytokine secretion, reactive oxygen intermediate (ROI) production and cytotoxic activity against respective colon cancer cells. Monocytes differentiated with TMVs exhibited morphological and phenotypical characteristics of macrophages. An early contact (beginning with the first day of the in vitro culture) of monocytes with TMVs resulted in increased IL-10 secretion and only slightly elevated TNF release. Early, or prolonged contact resulted in low ROI production and low cytotoxicity against tumour cells. On the other hand, late contact of MDM with TMVs, stimulated MDM to significant TNF and IL-12 secretion, ROI production and enhanced cytotoxicity against tumour cells in vitro. In addition, differences in MDM response to TMVs from different cell lines were observed (according to cytokine secretion, ROI production and cytotoxicity against tumour cells in vitro). Biological activity, STATs phosphorylation and microRNA profiling of MDMs indicated differences in their polarization/activation status which may suggest mixed polarization type M1/M2 with the predominance of proinflammatory cells after late contact with TMVs. Macrophage activity (polarization status) may be regulated by contact with not only tumour cells but also with TMVs. Their final polarization status depends on the contact time, and probably on the vesicle "cargo", as signified by the distinct impact of TMVs which enabled the switching of MDM maturation to regulatory macrophages.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 84 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 1%
Poland 1 1%
Unknown 82 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 20%
Researcher 14 17%
Student > Master 9 11%
Student > Bachelor 8 10%
Student > Doctoral Student 7 8%
Other 12 14%
Unknown 17 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 18 21%
Agricultural and Biological Sciences 17 20%
Medicine and Dentistry 15 18%
Immunology and Microbiology 9 11%
Pharmacology, Toxicology and Pharmaceutical Science 2 2%
Other 8 10%
Unknown 15 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 February 2016.
All research outputs
#14,246,461
of 22,842,950 outputs
Outputs from Journal of Translational Medicine
#1,783
of 3,997 outputs
Outputs of similar age
#208,284
of 397,125 outputs
Outputs of similar age from Journal of Translational Medicine
#28
of 78 outputs
Altmetric has tracked 22,842,950 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,997 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.5. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 397,125 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 78 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.