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Fusion cytokine IL-2-GMCSF enhances anticancer immune responses through promoting cell–cell interactions

Overview of attention for article published in Journal of Translational Medicine, February 2016
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  • Good Attention Score compared to outputs of the same age (68th percentile)

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1 patent

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31 Mendeley
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Title
Fusion cytokine IL-2-GMCSF enhances anticancer immune responses through promoting cell–cell interactions
Published in
Journal of Translational Medicine, February 2016
DOI 10.1186/s12967-016-0799-7
Pubmed ID
Authors

Qian Wen, Wenjing Xiong, Jianchun He, Shimeng Zhang, Xialin Du, Sudong Liu, Juanjuan Wang, Mingqian Zhou, Li Ma

Abstract

Potent antitumor responses can be induced through cytokine immunotherapy. Interleukin (IL)-2 and granulocyte-macrophage colony-stimulating factor (GM-CSF) are among the most effective cytokines to induce tumor-specific systemic immune responses and can act synergistically. To overcome the limitations of combined use of these two cytokines, we have constructed an IL2-GMCSF fusion protein and characterized its antitumor effects in this study. The expression of IL-2 receptor and GM-CSF receptor of cell lines were detected with quantitative real-time PCR. On this basis, the bioactivities of IL2-GMCSF, especially effects on DC2.4 cells were assayed. Another function of IL2-GMCSF-bridge two types of cells-was assessed by cell contact counting and cytotoxicity assays. The anti-tumor activity in vivo of IL2-GMCSF was evaluated in the melanoma model. The statistical significance among treatment groups were determined by One-Way ANOVA. The fusion protein IL2-GMCSF maintained the activities of IL-2 and GM-CSF, and could significantly promote DC2.4 cell activities, including phagocytosis, proliferation and cytokine secretion. In addition to the inherent cytokine activity, IL2-GMCSF bridges direct cell-cell interactions and enhances splenocyte killing efficacy against multiple tumor cell lines in vitro. Co-injection of IL2-GMCSF and inactivated B16F10 mouse melanoma cells induced complete immunoprotective responses in about 30 % of mice. These results suggested that IL2-GMCSF can efficiently regulate immune responses against tumors. Furthermore, as the bridging effect relies on both IL-2R and GM-CSFR and promotes interactions between immune and tumor cells, IL2-GMCSF may be utilized as a useful tool for dissecting specific immune responses for future clinical applications.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 26%
Student > Master 5 16%
Student > Bachelor 4 13%
Librarian 2 6%
Student > Postgraduate 2 6%
Other 3 10%
Unknown 7 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 29%
Biochemistry, Genetics and Molecular Biology 4 13%
Immunology and Microbiology 3 10%
Computer Science 2 6%
Veterinary Science and Veterinary Medicine 1 3%
Other 4 13%
Unknown 8 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 November 2020.
All research outputs
#6,053,278
of 19,280,406 outputs
Outputs from Journal of Translational Medicine
#948
of 3,422 outputs
Outputs of similar age
#109,304
of 359,817 outputs
Outputs of similar age from Journal of Translational Medicine
#1
of 1 outputs
Altmetric has tracked 19,280,406 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 3,422 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.3. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 359,817 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them