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Growth factor release by vesicular phospholipid gels: in-vitro results and application for rotator cuff repair in a rat model

Overview of attention for article published in BMC Musculoskeletal Disorders, April 2015
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Title
Growth factor release by vesicular phospholipid gels: in-vitro results and application for rotator cuff repair in a rat model
Published in
BMC Musculoskeletal Disorders, April 2015
DOI 10.1186/s12891-015-0542-1
Pubmed ID
Authors

Stefan Buchmann, Gunther H Sandmann, Lars Walz, Thomas Reichel, Knut Beitzel, Gabriele Wexel, Weiwei Tian, Achim Battmann, Stephan Vogt, Gerhard Winter, Andreas B Imhoff

Abstract

Biological augmentation of rotator cuff repair is of growing interest to improve biomechanical properties and prevent re-tearing. But intraoperative single shot growth factor application appears not sufficient to provide healing support in the physiologic growth factor expression peaks. The purpose of this study was to establish a sustained release of granulocyte-colony stimulating factor (G-CSF) from injectable vesicular phospholipid gels (VPGs) in vitro and to examine biocompatibility and influence on histology and biomechanical behavior of G-CSF loaded VPGs in a chronic supraspinatus tear rat model. G-CSF loaded VPGs were produced by dual asymmetric centrifugation. In vitro the integrity, stability and release rate were analyzed. In vivo supraspinatus tendons of 60 rats were detached and after 3 weeks a transosseous refixation with G-CSF loaded VPGs augmentation (n = 15; control, placebo, 1 and 10 μg G-CSF/d) was performed. 6 weeks postoperatively the healing site was analyzed histologically (n = 9; H&E by modified MOVIN score/Collagen I/III) and biomechanically (n = 6). In vitro testing revealed stable proteins after centrifugation and a continuous G-CSF release of up to 4 weeks. Placebo VPGs showed histologically no negative side effects on the healing process. Histologically in vivo testing demonstrated significant advantages for G-CSF 1 μg/d but not for G-CSF 10 μg/d in Collagen III content (p = 0.035) and a higher Collagen I/III ratio compared to the other groups. Biomechanically G-CSF 1 μg/d revealed a significant higher load to failure ratio (p = 0.020) compared to control but no significant differences in stiffness. By use of VPGs a continuous growth factor release could be obtained in vitro. The in vivo results demonstrate an improvement of immunohistology and biomechanical properties with a low dose G-CSF application via VPG. The VPG itself was well tolerated and had no negative influence on the healing behavior. Due to the favorable properties (highly adhesive, injectable, biocompatible) VPGs are a very interesting option for biologic augmentation. The study may serve as basis for further research in growth factor application models.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 2%
Unknown 43 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 9 20%
Student > Ph. D. Student 6 14%
Researcher 5 11%
Other 4 9%
Student > Bachelor 4 9%
Other 12 27%
Unknown 4 9%
Readers by discipline Count As %
Medicine and Dentistry 19 43%
Pharmacology, Toxicology and Pharmaceutical Science 5 11%
Engineering 4 9%
Nursing and Health Professions 4 9%
Agricultural and Biological Sciences 2 5%
Other 3 7%
Unknown 7 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 February 2016.
All research outputs
#3,549,599
of 7,103,144 outputs
Outputs from BMC Musculoskeletal Disorders
#1,153
of 1,929 outputs
Outputs of similar age
#167,319
of 319,351 outputs
Outputs of similar age from BMC Musculoskeletal Disorders
#43
of 84 outputs
Altmetric has tracked 7,103,144 research outputs across all sources so far. This one is in the 28th percentile – i.e., 28% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,929 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 319,351 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 84 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.