↓ Skip to main content

Classical scrapie prions are associated with peripheral blood monocytes and T-lymphocytes from naturally infected sheep

Overview of attention for article published in BMC Veterinary Research, February 2016
Altmetric Badge

About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (62nd percentile)
  • Good Attention Score compared to outputs of the same age and source (69th percentile)

Mentioned by

twitter
4 tweeters

Citations

dimensions_citation
10 Dimensions

Readers on

mendeley
23 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Classical scrapie prions are associated with peripheral blood monocytes and T-lymphocytes from naturally infected sheep
Published in
BMC Veterinary Research, February 2016
DOI 10.1186/s12917-016-0651-6
Pubmed ID
Authors

Rohana P. Dassanayake, Sally A. Madsen-Bouterse, Thomas C. Truscott, Dongyue Zhuang, Michelle R. Mousel, William C. Davis, David A. Schneider

Abstract

Classical scrapie is a transmissible spongiform encephalopathy (TSE) that affects sheep and goats. Our previous bioassay studies in lambs revealed that scrapie prions could be detected in association with peripheral blood monocular cells (PBMC), B lymphocytes and platelet-rich plasma fractions. In the present study, bioassay in lambs was again used to determine if scrapie prions are associated with the other two subsets of PBMC, monocytes and T lymphocytes. PBMC, monocytes and T lymphocytes were isolated from two preclinically affected VRQ/VRQ sheep naturally infected with classical ovine scrapie and intravenously transfused into VRQ/VRQ lambs post-weaning. As determined using standard immunohistochemistry for scrapie, abnormal isoforms of prion protein were detected in lymphoid tissues of lambs inoculated with PBMC (4/4 recipient lambs), monocytes (2/5) and T lymphocytes (1/4). Prion protein misfolding activity was detected by serial protein misfolding cyclic amplification (sPMCA) in PBMC from monocyte and T lymphocyte recipient sheep in agreement with antemortem rectal biopsy results, but such prion protein misfolding activity was not detected from other recipients. These findings show that scrapie prions are associated with monocytes and T lymphocytes circulating in the peripheral blood of sheep naturally infected with classical scrapie. Combined with our previous findings, we can now conclude that all three major subsets of PBMC can harbor prions during preclinical disease and thus, present logical targets for development of a sensitive assay to detect scrapie prions. In this regard, we have also demonstrated that sPMCA can be used to detect scrapie prions associated with PBMC.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 22%
Other 3 13%
Student > Bachelor 3 13%
Professor 2 9%
Student > Postgraduate 2 9%
Other 5 22%
Unknown 3 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 30%
Biochemistry, Genetics and Molecular Biology 5 22%
Veterinary Science and Veterinary Medicine 5 22%
Medicine and Dentistry 2 9%
Computer Science 1 4%
Other 0 0%
Unknown 3 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 February 2016.
All research outputs
#2,887,657
of 7,211,484 outputs
Outputs from BMC Veterinary Research
#274
of 1,082 outputs
Outputs of similar age
#118,964
of 320,257 outputs
Outputs of similar age from BMC Veterinary Research
#14
of 49 outputs
Altmetric has tracked 7,211,484 research outputs across all sources so far. This one has received more attention than most of these and is in the 59th percentile.
So far Altmetric has tracked 1,082 research outputs from this source. They receive a mean Attention Score of 2.3. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 320,257 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.
We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.