↓ Skip to main content

Use of the viral 2A peptide for bicistronic expression in transgenic mice

Overview of attention for article published in BMC Biology, September 2008
Altmetric Badge

Mentioned by

blogs
1 blog
twitter
1 X user
patent
14 patents
f1000
1 research highlight platform

Citations

dimensions_citation
196 Dimensions

Readers on

mendeley
534 Mendeley
citeulike
2 CiteULike
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Use of the viral 2A peptide for bicistronic expression in transgenic mice
Published in
BMC Biology, September 2008
DOI 10.1186/1741-7007-6-40
Pubmed ID
Authors

Georgios Trichas, Jo Begbie, Shankar Srinivas

Abstract

Transgenic animals are widely used in biomedical research and biotechnology. Multicistronic constructs, in which several proteins are encoded by a single messenger RNA, are commonly used in genetically engineered animals. This is currently done by using an internal ribosomal entry site to separate the different coding regions. 2A peptides result in the co-translational 'cleavage' of proteins and are an attractive alternative to the internal ribosomal entry site. They are more reliable than the internal ribosomal entry site and lead to expression of multiple cistrons at equimolar levels. They work in a wide variety of eukaryotic cells, but to date have not been demonstrated to function in transgenic mice in an inheritable manner.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 534 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 6 1%
United States 6 1%
United Kingdom 3 <1%
Spain 2 <1%
Japan 2 <1%
Belgium 2 <1%
Brazil 1 <1%
Israel 1 <1%
Netherlands 1 <1%
Other 8 1%
Unknown 502 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 135 25%
Student > Ph. D. Student 133 25%
Student > Master 50 9%
Student > Bachelor 43 8%
Professor > Associate Professor 33 6%
Other 80 15%
Unknown 60 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 270 51%
Biochemistry, Genetics and Molecular Biology 108 20%
Medicine and Dentistry 29 5%
Neuroscience 19 4%
Engineering 11 2%
Other 32 6%
Unknown 65 12%