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ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases

Overview of attention for article published in BMC Microbiology, July 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (92nd percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Citations

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809 Dimensions

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1546 Mendeley
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7 CiteULike
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Title
ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases
Published in
BMC Microbiology, July 2010
DOI 10.1186/1471-2180-10-189
Pubmed ID
Authors

Eva Bellemain, Tor Carlsen, Christian Brochmann, Eric Coissac, Pierre Taberlet, Håvard Kauserud

Abstract

During the last 15 years the internal transcribed spacer (ITS) of nuclear DNA has been used as a target for analyzing fungal diversity in environmental samples, and has recently been selected as the standard marker for fungal DNA barcoding. In this study we explored the potential amplification biases that various commonly utilized ITS primers might introduce during amplification of different parts of the ITS region in samples containing mixed templates ('environmental barcoding'). We performed in silico PCR analyses with commonly used primer combinations using various ITS datasets obtained from public databases as templates.

X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1,546 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 17 1%
France 6 <1%
Brazil 5 <1%
Canada 4 <1%
Australia 4 <1%
Germany 3 <1%
United Kingdom 3 <1%
India 3 <1%
Italy 2 <1%
Other 26 2%
Unknown 1473 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 289 19%
Researcher 268 17%
Student > Master 228 15%
Student > Bachelor 180 12%
Student > Doctoral Student 79 5%
Other 214 14%
Unknown 288 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 727 47%
Biochemistry, Genetics and Molecular Biology 189 12%
Environmental Science 121 8%
Immunology and Microbiology 58 4%
Medicine and Dentistry 28 2%
Other 102 7%
Unknown 321 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 August 2021.
All research outputs
#2,168,809
of 25,371,288 outputs
Outputs from BMC Microbiology
#111
of 3,489 outputs
Outputs of similar age
#7,680
of 104,617 outputs
Outputs of similar age from BMC Microbiology
#2
of 25 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,489 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 104,617 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.