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ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases

Overview of attention for article published in BMC Microbiology, January 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (93rd percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Citations

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630 Dimensions

Readers on

mendeley
1316 Mendeley
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7 CiteULike
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Title
ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases
Published in
BMC Microbiology, January 2010
DOI 10.1186/1471-2180-10-189
Pubmed ID
Authors

Eva Bellemain, Tor Carlsen, Christian Brochmann, Eric Coissac, Pierre Taberlet, Håvard Kauserud

Abstract

During the last 15 years the internal transcribed spacer (ITS) of nuclear DNA has been used as a target for analyzing fungal diversity in environmental samples, and has recently been selected as the standard marker for fungal DNA barcoding. In this study we explored the potential amplification biases that various commonly utilized ITS primers might introduce during amplification of different parts of the ITS region in samples containing mixed templates ('environmental barcoding'). We performed in silico PCR analyses with commonly used primer combinations using various ITS datasets obtained from public databases as templates.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 1,316 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 17 1%
Brazil 6 <1%
France 6 <1%
Canada 4 <1%
Australia 4 <1%
Germany 3 <1%
India 3 <1%
United Kingdom 3 <1%
Chile 2 <1%
Other 27 2%
Unknown 1241 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 269 20%
Researcher 245 19%
Student > Master 215 16%
Student > Bachelor 156 12%
Student > Doctoral Student 77 6%
Other 193 15%
Unknown 161 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 688 52%
Biochemistry, Genetics and Molecular Biology 160 12%
Environmental Science 110 8%
Immunology and Microbiology 46 3%
Medicine and Dentistry 26 2%
Other 95 7%
Unknown 191 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 August 2021.
All research outputs
#1,752,596
of 21,792,010 outputs
Outputs from BMC Microbiology
#97
of 3,077 outputs
Outputs of similar age
#15,344
of 252,292 outputs
Outputs of similar age from BMC Microbiology
#1
of 115 outputs
Altmetric has tracked 21,792,010 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,077 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,292 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 115 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.