Title |
Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method
|
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Published in |
Plant Methods, November 2009
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DOI | 10.1186/1746-4811-5-16 |
Pubmed ID | |
Authors |
Fu-Hui Wu, Shu-Chen Shen, Lan-Ying Lee, Shu-Hong Lee, Ming-Tsar Chan, Choun-Sea Lin |
Abstract |
Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein interactions. This method requires cutting leaves into very thin slivers to collect mesophyll cell protoplasts, a procedure that often causes cell damage, may yield only a few good protoplasts, and is time consuming. In addition, this protoplast isolation method normally requires a large number of leaves derived from plants grown specifically under low-light conditions, which may be a concern when material availability is limited such as with mutant plants, or in large scale experiments. |
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United States | 4 | 13% |
Germany | 4 | 13% |
France | 3 | 10% |
India | 1 | 3% |
United Kingdom | 1 | 3% |
Netherlands | 1 | 3% |
Poland | 1 | 3% |
Spain | 1 | 3% |
Finland | 1 | 3% |
Other | 0 | 0% |
Unknown | 14 | 45% |
Demographic breakdown
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Members of the public | 15 | 48% |
Scientists | 15 | 48% |
Science communicators (journalists, bloggers, editors) | 1 | 3% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Australia | 3 | <1% |
France | 2 | <1% |
Taiwan | 2 | <1% |
Brazil | 2 | <1% |
Belgium | 2 | <1% |
United States | 2 | <1% |
United Kingdom | 2 | <1% |
Italy | 1 | <1% |
Other | 9 | 1% |
Unknown | 750 | 96% |
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Student > Ph. D. Student | 193 | 25% |
Researcher | 155 | 20% |
Student > Master | 104 | 13% |
Student > Bachelor | 76 | 10% |
Student > Doctoral Student | 31 | 4% |
Other | 89 | 11% |
Unknown | 130 | 17% |
Readers by discipline | Count | As % |
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Chemistry | 9 | 1% |
Medicine and Dentistry | 5 | <1% |
Environmental Science | 4 | <1% |
Other | 18 | 2% |
Unknown | 145 | 19% |