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Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method

Overview of attention for article published in Plant Methods, November 2009
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (95th percentile)

Citations

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733 Dimensions

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mendeley
778 Mendeley
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1 CiteULike
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Title
Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method
Published in
Plant Methods, November 2009
DOI 10.1186/1746-4811-5-16
Pubmed ID
Authors

Fu-Hui Wu, Shu-Chen Shen, Lan-Ying Lee, Shu-Hong Lee, Ming-Tsar Chan, Choun-Sea Lin

Abstract

Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein interactions. This method requires cutting leaves into very thin slivers to collect mesophyll cell protoplasts, a procedure that often causes cell damage, may yield only a few good protoplasts, and is time consuming. In addition, this protoplast isolation method normally requires a large number of leaves derived from plants grown specifically under low-light conditions, which may be a concern when material availability is limited such as with mutant plants, or in large scale experiments.

X Demographics

X Demographics

The data shown below were collected from the profiles of 31 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 778 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 3 <1%
Australia 3 <1%
France 2 <1%
Taiwan 2 <1%
Brazil 2 <1%
Belgium 2 <1%
United States 2 <1%
United Kingdom 2 <1%
Italy 1 <1%
Other 9 1%
Unknown 750 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 193 25%
Researcher 155 20%
Student > Master 104 13%
Student > Bachelor 76 10%
Student > Doctoral Student 31 4%
Other 89 11%
Unknown 130 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 434 56%
Biochemistry, Genetics and Molecular Biology 163 21%
Chemistry 9 1%
Medicine and Dentistry 5 <1%
Environmental Science 4 <1%
Other 18 2%
Unknown 145 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 20. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2024.
All research outputs
#1,899,187
of 25,589,756 outputs
Outputs from Plant Methods
#85
of 1,280 outputs
Outputs of similar age
#8,065
of 178,919 outputs
Outputs of similar age from Plant Methods
#1
of 2 outputs
Altmetric has tracked 25,589,756 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,280 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 178,919 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them