Title |
Efficiency of Xist-mediated silencing on autosomes is linked to chromosomal domain organisation
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Published in |
Epigenetics & Chromatin, May 2010
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DOI | 10.1186/1756-8935-3-10 |
Pubmed ID | |
Authors |
Y Amy Tang, Derek Huntley, Giovanni Montana, Andrea Cerase, Tatyana B Nesterova, Neil Brockdorff |
Abstract |
X chromosome inactivation, the mechanism used by mammals to equalise dosage of X-linked genes in XX females relative to XY males, is triggered by chromosome-wide localisation of a cis-acting non-coding RNA, Xist. The mechanism of Xist RNA spreading and Xist-dependent silencing is poorly understood. A large body of evidence indicates that silencing is more efficient on the X chromosome than on autosomes, leading to the idea that the X chromosome has acquired sequences that facilitate propagation of silencing. LINE-1 (L1) repeats are relatively enriched on the X chromosome and have been proposed as candidates for these sequences. To determine the requirements for efficient silencing we have analysed the relationship of chromosome features, including L1 repeats, and the extent of silencing in cell lines carrying inducible Xist transgenes located on one of three different autosomes. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 94 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Unknown | 10 | 11% |
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Immunology and Microbiology | 2 | 2% |
Other | 3 | 3% |
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