Title |
Characterisation of the dynamic behaviour of lipid droplets in the early mouse embryo using adaptive harmonic generation microscopy
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Published in |
BMC Molecular and Cell Biology, June 2010
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DOI | 10.1186/1471-2121-11-38 |
Pubmed ID | |
Authors |
Tomoko Watanabe, Anisha Thayil, Alexander Jesacher, Kate Grieve, Delphine Debarre, Tony Wilson, Martin Booth, Shankar Srinivas |
Abstract |
Lipid droplets (LD) are organelles with an important role in normal metabolism and disease. The lipid content of embryos has a major impact on viability and development. LD in Drosophila embryos and cultured cell lines have been shown to move and fuse in a microtubule dependent manner. Due to limitations in current imaging technology, little is known about the behaviour of LD in the mammalian embryo. Harmonic generation microscopy (HGM) allows one to image LD without the use of exogenous labels. Adaptive optics can be used to correct aberrations that would otherwise degrade the quality and information content of images. |
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Demographic breakdown
Readers by professional status | Count | As % |
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Other | 20 | 24% |
Unknown | 8 | 10% |
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Veterinary Science and Veterinary Medicine | 4 | 5% |
Other | 10 | 12% |
Unknown | 9 | 11% |