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Comparison and optimization of ten phage encoded serine integrases for genome engineering in Saccharomyces cerevisiae

Overview of attention for article published in BMC Biotechnology, February 2016
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  • Above-average Attention Score compared to outputs of the same age and source (51st percentile)

Mentioned by

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2 tweeters

Citations

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Readers on

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69 Mendeley
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Title
Comparison and optimization of ten phage encoded serine integrases for genome engineering in Saccharomyces cerevisiae
Published in
BMC Biotechnology, February 2016
DOI 10.1186/s12896-016-0241-5
Pubmed ID
Authors

Zhengyao Xu, William R. A. Brown

Abstract

Phage-encoded serine integrases, such as ϕC31 integrase, are widely used for genome engineering but have not been optimized for use in Saccharomyces cerevisiae although this organism is a widely used organism in biotechnology. The activities of derivatives of fourteen serine integrases that either possess or lack a nuclear localization signal were compared using a standardized recombinase mediated cassette exchange reaction. The relative activities of these integrases in S. cerevisiae and in mammalian cells suggested that the major determinant of the activity of an integrase is the enzyme itself and not the cell in which it is working. We used an inducible promoter to show that six integrases were toxic as judged by their effects upon the proliferative ability of transformed yeast. We show that in general the active phage-encoded serine integrases were an order of magnitude more efficient in promoting genome integration reactions than a simple homologous recombination. The results of our study allow us to identify the integrases of the phage ϕBT1, TP901 ~ nls, R4, Bxb1, MR11, A118, ϕK38, ϕC31 ~ nls, Wβ and SPBC ~ nls as active in S. cerevisiae and indicate that vertebrate cells are more restricted than yeast in terms of which integrases are active.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 69 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 20 29%
Student > Ph. D. Student 16 23%
Student > Bachelor 11 16%
Student > Master 8 12%
Professor 1 1%
Other 4 6%
Unknown 9 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 25 36%
Agricultural and Biological Sciences 22 32%
Chemical Engineering 3 4%
Immunology and Microbiology 3 4%
Medicine and Dentistry 2 3%
Other 6 9%
Unknown 8 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2016.
All research outputs
#3,648,173
of 7,216,446 outputs
Outputs from BMC Biotechnology
#267
of 458 outputs
Outputs of similar age
#169,110
of 320,632 outputs
Outputs of similar age from BMC Biotechnology
#12
of 27 outputs
Altmetric has tracked 7,216,446 research outputs across all sources so far. This one is in the 28th percentile – i.e., 28% of other outputs scored the same or lower than it.
So far Altmetric has tracked 458 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 320,632 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.