↓ Skip to main content

Development of a novel 96-microwell assay with high throughput for determination of olmesartan medoxomil in its tablets

Overview of attention for article published in BMC Chemistry, January 2012
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
140 Dimensions

Readers on

mendeley
12 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Development of a novel 96-microwell assay with high throughput for determination of olmesartan medoxomil in its tablets
Published in
BMC Chemistry, January 2012
DOI 10.1186/1752-153x-6-1
Pubmed ID
Authors

Ibrahim A Darwish, Tanveer A Wani, Nasr Y Khalil, Abdul-Aziz Al-Shaikh, Najm Al-Morshadi

Abstract

A novel 96-microwell-based spectrophotometric assay has been developed and validated for determination of olmesartan medoxomil (OLM) in tablets. The formation of a colored charge-transfer (CT) complex between OLM as a n-electron donor and 2, 5-dichloro-3, 6-dihydroxy-1, 4-benzoquinone (p-chloranilic acid, pCA) as a π-electron acceptor was investigated, for the first time, and employed as a basis in the development of the proposed assay. The proposed assay was carried out in 96-microwell plates. The absorbance of the colored-CT complex was measured at 490 nm by microwell-plate absorbance reader. The optimum conditions of the reaction and the analytical procedures of the assay were established. Under the optimum conditions, linear relationship with good correlation coefficient was found between the absorbance and the concentration of OLM in the range of 1-200 μg ml-1. The limits of detection and quantitation were 0.3 and 1 μg ml-1, respectively. No interference was observed from the additives that are present in the pharmaceutical formulation or from hydrochlorothiazide and amlodipine that are co-formulated with OLM in some formulations. The assay was successfully applied to the analysis of OLM in tablets with good accuracy and precision. The assay described herein has great practical value in the routine analysis of OLM in quality control laboratories, as it has high throughput property, consumes minimum volume of organic solvent thus it offers the reduction in the exposures of the analysts to the toxic effects of organic solvents, and reduction in the analysis cost by 50-fold. Although the proposed assay was validated for OLM, however, the same methodology could be used for any electron-donating analyte for which a CT reaction can be performed.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 33%
Student > Ph. D. Student 3 25%
Professor > Associate Professor 2 17%
Student > Bachelor 1 8%
Professor 1 8%
Other 0 0%
Unknown 1 8%
Readers by discipline Count As %
Chemistry 5 42%
Medicine and Dentistry 2 17%
Agricultural and Biological Sciences 1 8%
Pharmacology, Toxicology and Pharmaceutical Science 1 8%
Biochemistry, Genetics and Molecular Biology 1 8%
Other 1 8%
Unknown 1 8%