Title |
LIN7A is a major determinant of cell-polarity defects in breast carcinomas
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Published in |
Breast Cancer Research, February 2016
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DOI | 10.1186/s13058-016-0680-x |
Pubmed ID | |
Authors |
Nadège Gruel, Laetitia Fuhrmann, Catalina Lodillinsky, Vanessa Benhamo, Odette Mariani, Aurélie Cédenot, Laurent Arnould, Gaëtan Macgrogan, Xavier Sastre-Garau, Philippe Chavrier, Olivier Delattre, Anne Vincent-Salomon |
Abstract |
Polarity defects are a hallmark of most carcinomas. Cells from invasive micropapillary carcinomas (IMPCs) of the breast are characterized by a striking cell polarity inversion and represent an interesting model for the analysis of polarity abnormalities. In-depth investigation of polarity proteins in 24 IMPCs and a gene expression profiling, comparing IMPC (n = 73) with invasive carcinomas of no special type (ICNST) (n = 51) have been performed. IMPCs showed a profound disorganization of the investigated polarity proteins and revealed major abnormalities in their subcellular localization. Gene expression profiling experiments highlighted a number of deregulated genes in the IMPCs that have a role in apico-basal polarity, adhesion and migration. LIN7A, a Crumbs-complex polarity gene, was one of the most differentially over-expressed genes in the IMPCs. Upon LIN7A over-expression, we observed hyperproliferation, invasion and a complete absence of lumen formation, revealing strong polarity defects. This study therefore shows that LIN7A has a crucial role in the polarity abnormalities associated with breast carcinogenesis. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
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Germany | 1 | 7% |
Unknown | 14 | 93% |
Demographic breakdown
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Student > Ph. D. Student | 4 | 27% |
Student > Master | 2 | 13% |
Researcher | 2 | 13% |
Student > Doctoral Student | 1 | 7% |
Other | 1 | 7% |
Other | 1 | 7% |
Unknown | 4 | 27% |
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Biochemistry, Genetics and Molecular Biology | 4 | 27% |
Agricultural and Biological Sciences | 2 | 13% |
Unknown | 4 | 27% |