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Variation in 5-hydroxymethylcytosine across human cortex and cerebellum

Overview of attention for article published in Genome Biology (Online Edition), February 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (83rd percentile)

Mentioned by

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16 tweeters

Citations

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71 Dimensions

Readers on

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102 Mendeley
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Title
Variation in 5-hydroxymethylcytosine across human cortex and cerebellum
Published in
Genome Biology (Online Edition), February 2016
DOI 10.1186/s13059-016-0871-x
Pubmed ID
Authors

Katie Lunnon, Eilis Hannon, Rebecca G. Smith, Emma Dempster, Chloe Wong, Joe Burrage, Claire Troakes, Safa Al-Sarraj, Agnieszka Kepa, Leonard Schalkwyk, Jonathan Mill

Abstract

The most widely utilized approaches for quantifying DNA methylation involve the treatment of genomic DNA with sodium bisulfite; however, this method cannot distinguish between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Previous studies have shown that 5hmC is enriched in the brain, although little is known about its genomic distribution and how it differs between anatomical regions and individuals. In this study, we combine oxidative bisulfite (oxBS) treatment with the Illumina Infinium 450K BeadArray to quantify genome-wide patterns of 5hmC in two distinct anatomical regions of the brain from multiple individuals. We identify 37,145 and 65,563 sites passing our threshold for detectable 5hmC in the prefrontal cortex and cerebellum respectively, with 23,445 loci common across both brain regions. Distinct patterns of 5hmC are identified in each brain region, with notable differences in the genomic location of the most hydroxymethylated loci between these brain regions. Tissue-specific patterns of 5hmC are subsequently confirmed in an independent set of prefrontal cortex and cerebellum samples. This study represents the first systematic analysis of 5hmC in the human brain, identifying tissue-specific hydroxymethylated positions and genomic regions characterized by inter-individual variation in DNA hydroxymethylation. This study demonstrates the utility of combining oxBS-treatment with the Illumina 450k methylation array to systematically quantify 5hmC across the genome and the potential utility of this approach for epigenomic studies of brain disorders.

Twitter Demographics

The data shown below were collected from the profiles of 16 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 102 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 3%
Netherlands 1 <1%
Unknown 98 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 29 28%
Researcher 19 19%
Student > Master 8 8%
Student > Bachelor 8 8%
Other 6 6%
Other 21 21%
Unknown 11 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 29%
Biochemistry, Genetics and Molecular Biology 27 26%
Neuroscience 17 17%
Medicine and Dentistry 7 7%
Computer Science 1 <1%
Other 4 4%
Unknown 16 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 March 2016.
All research outputs
#2,128,211
of 16,566,910 outputs
Outputs from Genome Biology (Online Edition)
#1,770
of 3,490 outputs
Outputs of similar age
#43,180
of 268,198 outputs
Outputs of similar age from Genome Biology (Online Edition)
#1
of 1 outputs
Altmetric has tracked 16,566,910 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,490 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 26.3. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 268,198 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them