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Genomic and transcriptomic comparison of nucleotide variations for insights into bruchid resistance of mungbean (Vigna radiata [L.] R. Wilczek)

Overview of attention for article published in BMC Plant Biology, February 2016
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Title
Genomic and transcriptomic comparison of nucleotide variations for insights into bruchid resistance of mungbean (Vigna radiata [L.] R. Wilczek)
Published in
BMC Plant Biology, February 2016
DOI 10.1186/s12870-016-0736-1
Pubmed ID
Authors

Mao-Sen Liu, Tony Chien-Yen Kuo, Chia-Yun Ko, Dung-Chi Wu, Kuan-Yi Li, Wu-Jui Lin, Ching-Ping Lin, Yen-Wei Wang, Roland Schafleitner, Hsiao-Feng Lo, Chien-Yu Chen, Long-Fang O. Chen

Abstract

Mungbean (Vigna radiata [L.] R. Wilczek) is an important legume crop with high nutritional value in South and Southeast Asia. The crop plant is susceptible to a storage pest caused by bruchids (Callosobruchus spp.). Some wild and cultivated mungbean accessions show resistance to bruchids. Genomic and transcriptomic comparison of bruchid-resistant and -susceptible mungbean could reveal bruchid-resistant genes (Br) for this pest and give insights into the bruchid resistance of mungbean. Flow cytometry showed that the genome size varied by 61 Mb (mega base pairs) among the tested mungbean accessions. Next generation sequencing followed by de novo assembly of the genome of the bruchid-resistant recombinant inbred line 59 (RIL59) revealed more than 42,000 genes. Transcriptomic comparison of bruchid-resistant and -susceptible parental lines and their offspring identified 91 differentially expressed genes (DEGs) classified into 17 major and 74 minor bruchid-resistance-associated genes. We found 408 nucleotide variations (NVs) between bruchid-resistant and -susceptible lines in regions spanning 2 kb (kilo base pairs) of the promoters of 68 DEGs. Furthermore, 282 NVs were identified on exons of 148 sequence-changed-protein genes (SCPs). DEGs and SCPs comprised genes involved in resistant-related, transposable elements (TEs) and conserved metabolic pathways. A large number of these genes were mapped to a region on chromosome 5. Molecular markers designed for variants of putative bruchid-resistance-associated genes were highly diagnostic for the bruchid-resistant genotype. In addition to identifying bruchid-resistance-associated genes, we found that conserved metabolism and TEs may be modifier factors for bruchid resistance of mungbean. The genome sequence of a bruchid-resistant inbred line, candidate genes and sequence variations in promoter regions and exons putatively conditioning resistance as well as markers detecting these variants could be used for development of bruchid-resistant mungbean varieties.

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Geographical breakdown

Country Count As %
Unknown 33 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 24%
Researcher 5 15%
Student > Master 3 9%
Lecturer 2 6%
Unspecified 2 6%
Other 3 9%
Unknown 10 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 27%
Biochemistry, Genetics and Molecular Biology 8 24%
Unspecified 2 6%
Earth and Planetary Sciences 1 3%
Social Sciences 1 3%
Other 1 3%
Unknown 11 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2016.
All research outputs
#18,441,836
of 22,849,304 outputs
Outputs from BMC Plant Biology
#2,102
of 3,256 outputs
Outputs of similar age
#216,421
of 297,955 outputs
Outputs of similar age from BMC Plant Biology
#43
of 62 outputs
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