Title |
Increased sensitivity of next generation sequencing-based expression profiling after globin reduction in human blood RNA
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Published in |
BMC Genomics, January 2012
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DOI | 10.1186/1471-2164-13-28 |
Pubmed ID | |
Authors |
Anastasios Mastrokolias, Johan T den Dunnen, GertJan B van Ommen, Peter AC 't Hoen, Willeke MC van Roon-Mom |
Abstract |
Transcriptome analysis is of great interest in clinical research, where significant differences between individuals can be translated into biomarkers of disease. Although next generation sequencing provides robust, comparable and highly informative expression profiling data, with several million of tags per blood sample, reticulocyte globin transcripts can constitute up to 76% of total mRNA compromising the detection of low abundant transcripts. We have removed globin transcripts from 6 human whole blood RNA samples with a human globin reduction kit and compared them with the same non-reduced samples using deep Serial Analysis of Gene Expression. |
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United Kingdom | 2 | 50% |
France | 1 | 25% |
Germany | 1 | 25% |
Demographic breakdown
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Unknown | 1 | 25% |
Mendeley readers
Geographical breakdown
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United States | 4 | 4% |
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South Africa | 1 | <1% |
Brazil | 1 | <1% |
Unknown | 103 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 41 | 37% |
Student > Ph. D. Student | 20 | 18% |
Other | 9 | 8% |
Student > Master | 8 | 7% |
Professor > Associate Professor | 6 | 5% |
Other | 16 | 14% |
Unknown | 11 | 10% |
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Environmental Science | 2 | 2% |
Other | 11 | 10% |
Unknown | 16 | 14% |