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Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

Overview of attention for article published in Plant Methods, February 2016
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2 tweeters

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Title
Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana
Published in
Plant Methods, February 2016
DOI 10.1186/s13007-016-0116-8
Pubmed ID
Authors

Olaf Czarnecki, Anthony C. Bryan, Sara S. Jawdy, Xiaohan Yang, Zong-Ming Cheng, Jin-Gui Chen, Gerald A. Tuskan

Abstract

Genetic engineering of plants that results in successful establishment of new biochemical or regulatory pathways requires stable introduction of one or more genes into the plant genome. It might also be necessary to down-regulate or turn off expression of endogenous genes in order to reduce activity of competing pathways. An established way to knockdown gene expression in plants is expressing a hairpin-RNAi construct, eventually leading to degradation of a specifically targeted mRNA. Knockdown of multiple genes that do not share homologous sequences is still challenging and involves either sophisticated cloning strategies to create vectors with different serial expression constructs or multiple transformation events that is often restricted by a lack of available transformation markers. Synthetic RNAi fragments were assembled in yeast carrying homologous sequences to six or seven non-family genes and introduced into pAGRIKOLA. Transformation of Arabidopsis thaliana and subsequent expression analysis of targeted genes proved efficient knockdown of all target genes. We present a simple and cost-effective method to create constructs to simultaneously knockdown multiple non-family genes or genes that do not share sequence homology. The presented method can be applied in plant and animal synthetic biology as well as traditional plant and animal genetic engineering.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 68 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 15 22%
Student > Master 12 18%
Student > Ph. D. Student 12 18%
Student > Bachelor 10 15%
Other 2 3%
Other 5 7%
Unknown 12 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 37 54%
Biochemistry, Genetics and Molecular Biology 14 21%
Social Sciences 1 1%
Engineering 1 1%
Unknown 15 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2016.
All research outputs
#16,482,139
of 21,174,934 outputs
Outputs from Plant Methods
#842
of 1,000 outputs
Outputs of similar age
#186,389
of 278,605 outputs
Outputs of similar age from Plant Methods
#4
of 4 outputs
Altmetric has tracked 21,174,934 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,000 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 10th percentile – i.e., 10% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,605 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one.