@etotheipie @tgof137 @lostlineage @ydeigin @franciscodeasis “Our modified protocol.. allowed facile large single insertions, deletions/truncations and multiple mutations in a single experiment” Insertion by mutation. This was 2006 technology https://t.co
@CianDuggan I like using this method: https://t.co/4NEhvc1Msg It's like QuickChange but with non-overlapping 3' ends to the primers. It makes a whole lot more mutated DNA. But the primer design isn't super easy to understand from the paper and there may be
@AJPierik @CianDuggan Also this: https://t.co/WkzBJ180wx