Title |
Decrease of energy spilling in Escherichia coli continuous cultures with rising specific growth rate and carbon wasting
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Published in |
BMC Systems Biology, July 2011
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DOI | 10.1186/1752-0509-5-106 |
Pubmed ID | |
Authors |
Kaspar Valgepea, Kaarel Adamberg, Raivo Vilu |
Abstract |
Growth substrates, aerobic/anaerobic conditions, specific growth rate (μ) etc. strongly influence Escherichia coli cell physiology in terms of cell size, biomass composition, gene and protein expression. To understand the regulation behind these different phenotype properties, it is useful to know carbon flux patterns in the metabolic network which are generally calculated by metabolic flux analysis (MFA). However, rarely is biomass composition determined and carbon balance carefully measured in the same experiments which could possibly lead to distorted MFA results and questionable conclusions. Therefore, we carried out both detailed carbon balance and biomass composition analysis in the same experiments for more accurate quantitative analysis of metabolism and MFA. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Estonia | 3 | 3% |
United States | 2 | 2% |
Australia | 2 | 2% |
Sweden | 1 | <1% |
United Kingdom | 1 | <1% |
Germany | 1 | <1% |
Mexico | 1 | <1% |
France | 1 | <1% |
Denmark | 1 | <1% |
Other | 1 | <1% |
Unknown | 106 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 35 | 29% |
Researcher | 28 | 23% |
Student > Master | 13 | 11% |
Student > Bachelor | 12 | 10% |
Professor > Associate Professor | 7 | 6% |
Other | 17 | 14% |
Unknown | 8 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 48 | 40% |
Biochemistry, Genetics and Molecular Biology | 26 | 22% |
Engineering | 11 | 9% |
Chemical Engineering | 6 | 5% |
Environmental Science | 5 | 4% |
Other | 12 | 10% |
Unknown | 12 | 10% |