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Structure of a VHH isolated from a naïve phage display library

Overview of attention for article published in BMC Research Notes, March 2019
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Title
Structure of a VHH isolated from a naïve phage display library
Published in
BMC Research Notes, March 2019
DOI 10.1186/s13104-019-4197-0
Pubmed ID
Authors

Brandy White, Ian Huh, Cory L. Brooks

Abstract

To determine the X-ray structure and biophysical properties of a Camelid VHH isolated from a naïve phage display library. Single domain antibodies (VHH) derived from the unique immune system of the Camelidae family have gained traction as useful tools for biotechnology as well as a source of potentially novel therapeutics. Here we report the structure and biophysical characterization of a VHH originally isolated from a naïve camelid phage display library. VHH R419 has a melting temperate of 66 °C and was found to be a monomer in solution. The protein crystallized in space group P6522 and the structure was solved by molecular replacement to a resolution of 1.5 Å. The structure revealed a flat paratope with CDR loops that could be classified into existing canonical loop structures. A combination of high expression yield, stability and rapid crystallization might make R419 into a candidate scaffold for CDR grafting and homology modeling.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 22%
Student > Bachelor 5 16%
Student > Ph. D. Student 4 13%
Student > Master 3 9%
Other 2 6%
Other 1 3%
Unknown 10 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 28%
Veterinary Science and Veterinary Medicine 2 6%
Agricultural and Biological Sciences 2 6%
Engineering 2 6%
Chemistry 2 6%
Other 5 16%
Unknown 10 31%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 April 2019.
All research outputs
#11,578,171
of 14,613,233 outputs
Outputs from BMC Research Notes
#2,253
of 3,318 outputs
Outputs of similar age
#196,201
of 266,053 outputs
Outputs of similar age from BMC Research Notes
#1
of 1 outputs
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