Title |
Assessment of yeast Saccharomyces cerevisiae component binding to Mycobacterium avium subspecies paratuberculosis using bovine epithelial cells
|
---|---|
Published in |
BMC Veterinary Research, March 2016
|
DOI | 10.1186/s12917-016-0665-0 |
Pubmed ID | |
Authors |
Ziwei Li, Qiumei You, Faisury Ossa, Philip Mead, Margaret Quinton, Niel A. Karrow |
Abstract |
Since yeast Saccharomyces cerevisiae and its components are being used for the prevention and treatment of enteric diseases in different species, they may also be useful for preventing Johne's disease, a chronic inflammatory bowel disease of ruminants caused by Mycobacterium avium spp. paratuberculosis (MAP). This study aimed to identify potential yeast derivatives that may be used to help prevent MAP infection. The adherence of mCherry-labeled MAP to bovine mammary epithelial cell line (MAC-T cells) and bovine primary epithelial cells (BECs) co-cultured with yeast cell wall components (CWCs) from four different yeast strains (A, B, C and D) and two forms of dead yeast from strain A was investigated. The CWCs from all four yeast strains and the other two forms of dead yeast from strain A reduced MAP adhesion to MAC-T cells and BECs in a concentration-dependent manner after 6-h of exposure, with the dead yeast having the greatest effect. The following in vitro binding studies suggest that dead yeast and its' CWCs may be useful for reducing risk of MAP infection. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 25 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 5 | 20% |
Researcher | 5 | 20% |
Student > Bachelor | 3 | 12% |
Student > Ph. D. Student | 2 | 8% |
Student > Doctoral Student | 2 | 8% |
Other | 2 | 8% |
Unknown | 6 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 24% |
Veterinary Science and Veterinary Medicine | 4 | 16% |
Medicine and Dentistry | 2 | 8% |
Biochemistry, Genetics and Molecular Biology | 1 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 4 | 16% |
Unknown | 7 | 28% |