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Restriction of HIV-1-based lentiviral vectors in adult primary marrow-derived and peripheral mobilized human CD34+ hematopoietic stem and progenitor cells occurs prior to viral DNA integration

Overview of attention for article published in Retrovirology, March 2016
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (73rd percentile)

Mentioned by

twitter
4 tweeters
wikipedia
1 Wikipedia page

Citations

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6 Dimensions

Readers on

mendeley
26 Mendeley
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Title
Restriction of HIV-1-based lentiviral vectors in adult primary marrow-derived and peripheral mobilized human CD34+ hematopoietic stem and progenitor cells occurs prior to viral DNA integration
Published in
Retrovirology, March 2016
DOI 10.1186/s12977-016-0246-0
Pubmed ID
Authors

Daniel O. Griffin, Stephen P. Goff

Abstract

Gene therapy is currently being attempted using a number of delivery vehicles including lentiviral-based vectors. The delivery and insertion of a gene using lentiviral-based vectors involves multiple discrete steps, including reverse transcription of viral RNA into DNA, nuclear entry, integration of viral DNA into the host genome and expression of integrated genes. Transduction of murine stem cells by the murine leukemia viruses is inefficient because the expression of the integrated DNA is profoundly blocked. Transduction of human stem cells by lentivirus vectors is also inefficient, but the cause and specific part of the retroviral lifecycle where this block occurs is unknown. Here we demonstrate that the dominant point of restriction of an HIV-1-based lentiviral vector in adult human hematopoietic stem and progenitor cells (HSPCs) from bone marrow and also those obtained following peripheral mobilization is prior to viral DNA integration. We specifically show that restriction of HSPCs to an HIV-1-based lentiviral vector is prior to formation of nuclear DNA forms. Murine restriction of MLV and human cellular restriction of HIV-1 are fundamentally different. While murine restriction of MLV occurs post integration, human restriction of HIV-1 occurs before integration.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 4%
Denmark 1 4%
Unknown 24 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 42%
Researcher 5 19%
Student > Bachelor 2 8%
Other 2 8%
Student > Doctoral Student 1 4%
Other 2 8%
Unknown 3 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 38%
Biochemistry, Genetics and Molecular Biology 3 12%
Medicine and Dentistry 3 12%
Immunology and Microbiology 3 12%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Other 3 12%
Unknown 3 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2020.
All research outputs
#4,490,096
of 17,429,432 outputs
Outputs from Retrovirology
#231
of 928 outputs
Outputs of similar age
#71,740
of 271,971 outputs
Outputs of similar age from Retrovirology
#1
of 1 outputs
Altmetric has tracked 17,429,432 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 928 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 271,971 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them