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Comparative study of equine mesenchymal stem cells from healthy and injured synovial tissues: an in vitro assessment

Overview of attention for article published in Stem Cell Research & Therapy, March 2016
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Title
Comparative study of equine mesenchymal stem cells from healthy and injured synovial tissues: an in vitro assessment
Published in
Stem Cell Research & Therapy, March 2016
DOI 10.1186/s13287-016-0294-3
Pubmed ID
Authors

Joice Fülber, Durvanei A. Maria, Luis Cláudio Lopes Correia da Silva, Cristina O. Massoco, Fernanda Agreste, Raquel Y. Arantes Baccarin

Abstract

Bone marrow and adipose tissues are known sources of mesenchymal stem cells (MSCs) in horses; however, synovial tissues might be a promising alternative. The aim of this study was to evaluate phenotypic characteristics and differentiation potential of equine MSCs from synovial fluid (SF) and synovial membrane (SM) of healthy joints (SF-H and SM-H), joints with osteoarthritis (SF-OA and SM-OA) and joints with osteochondritis dissecans (SF-OCD and SM-OCD) to determine the most suitable synovial source for an allogeneic therapy cell bank. Expression of the markers CD90, CD105, CD44, and CD34 in SF-H, SM-H, SF-OA, SM-OA, SF-OCD and SM-OCD was verified by flow cytometry, and expression of cytokeratin, vimentin, PGP 9.5, PCNA, lysozyme, nanog, and Oct4 was verified by immunocytochemistry. MSCs were cultured and evaluated for their chondrogenic, osteogenic and adipogenic differentiation potential. Final quantification of extracellular matrix and mineralized matrix was determined using AxioVision software. A tumorigenicity test was conducted in Balb-C(nu/nu) mice to verify the safety of the MSCs from these sources. Cultured cells from SF and SM exhibited fibroblastoid morphology and the ability to adhere to plastic. The time elapsed between primary culture and the third passage was approximately 73 days for SF-H, 89 days for SF-OCD, 60 days for SF-OA, 68 days for SM-H, 57 days for SM-OCD and 54 days for SM-OA. The doubling time for SF-OCD was higher than that for other cells at the first passage (P < 0.05). MSCs from synovial tissues showed positive expression of the markers CD90, CD44, lysozyme, PGP 9.5, PCNA and vimentin and were able to differentiate into chondrogenic (21 days) and osteogenic (21 days) lineages, and, although poorly, into adipogenic lineages (14 days). The areas staining positive for extracellular matrix in the SF-H and SM-H groups were larger than those in the SF-OA and SM-OA groups (P < 0.05). The positive mineralized matrix area in the SF-H group was larger than those in all the other groups (P < 0.05). The studied cells exhibited no tumorigenic effects. SF and SM are viable sources of equine MSCs. All sources studied provide suitable MSCs for an allogeneic therapy cell bank; nevertheless, MSCs from healthy joints may be preferable for cell banking purposes because they exhibit better chondrogenic differentiation capacity.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 58 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 16%
Student > Bachelor 8 14%
Student > Doctoral Student 8 14%
Student > Master 8 14%
Student > Ph. D. Student 5 9%
Other 10 17%
Unknown 10 17%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 11 19%
Biochemistry, Genetics and Molecular Biology 9 16%
Medicine and Dentistry 9 16%
Agricultural and Biological Sciences 6 10%
Engineering 4 7%
Other 6 10%
Unknown 13 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 March 2016.
All research outputs
#3,774,798
of 7,361,309 outputs
Outputs from Stem Cell Research & Therapy
#328
of 558 outputs
Outputs of similar age
#151,622
of 280,306 outputs
Outputs of similar age from Stem Cell Research & Therapy
#23
of 39 outputs
Altmetric has tracked 7,361,309 research outputs across all sources so far. This one is in the 28th percentile – i.e., 28% of other outputs scored the same or lower than it.
So far Altmetric has tracked 558 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,306 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 39 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.