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Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS

Overview of attention for article published in BMC Microbiology, March 2016
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  • Above-average Attention Score compared to outputs of the same age (55th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

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3 tweeters

Citations

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87 Mendeley
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Title
Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS
Published in
BMC Microbiology, March 2016
DOI 10.1186/s12866-016-0643-5
Pubmed ID
Authors

Kristina Majsec, Edward L. Bolt, Ivana Ivančić-Baće

Abstract

CRISPR-Cas systems provide adaptive immunity to mobile genetic elements in prokaryotes. In many bacteria, including E. coli, a specialized ribonucleoprotein complex called Cascade enacts immunity by" an interference reaction" between CRISPR encoded RNA (crRNA) and invader DNA sequences called "protospacers". Cascade recognizes invader DNA via short "protospacer adjacent motif" (PAM) sequences and crRNA-DNA complementarity. This triggers degradation of invader DNA by Cas3 protein and in some circumstances stimulates capture of new invader DNA protospacers for incorporation into CRISPR as "spacers" by Cas1 and Cas2 proteins, thus enhancing immunity. Co-expression of Cascade, Cas3 and crRNA is effective at giving E. coli cells resistance to phage lysis, if a transcriptional repressor of Cascade and CRISPR, H-NS, is inactivated (Δhns). We present further genetic analyses of the regulation of CRISPR-Cas mediated phage resistance in Δhns E. coli cells. We observed that E. coli Type I-E CRISPR-Cas mediated resistance to phage λ was strongly temperature dependent, when repeating previously published experimental procedures. Further genetic analyses highlighted the importance of culture conditions for controlling the extent of CRISPR immunity in E. coli. These data identified that expression levels of cas3 is an important limiting factor for successful resistance to phage. Significantly, we describe the new identification that cas3 is also under transcriptional control by H-NS but that this is exerted only in stationary phase cells. Regulation of cas3 is responsive to phase of growth, and to growth temperature in E. coli, impacting on the efficacy of CRISPR-Cas immunity in these experimental systems.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 87 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 22%
Student > Bachelor 14 16%
Student > Master 12 14%
Researcher 11 13%
Professor > Associate Professor 5 6%
Other 12 14%
Unknown 14 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 32 37%
Agricultural and Biological Sciences 27 31%
Immunology and Microbiology 4 5%
Engineering 3 3%
Computer Science 2 2%
Other 7 8%
Unknown 12 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 March 2016.
All research outputs
#3,246,664
of 7,377,175 outputs
Outputs from BMC Microbiology
#472
of 1,253 outputs
Outputs of similar age
#120,488
of 279,079 outputs
Outputs of similar age from BMC Microbiology
#28
of 63 outputs
Altmetric has tracked 7,377,175 research outputs across all sources so far. This one has received more attention than most of these and is in the 55th percentile.
So far Altmetric has tracked 1,253 research outputs from this source. They receive a mean Attention Score of 2.9. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,079 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 63 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.