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A simple, accurate and universal method for quantification of PCR

Overview of attention for article published in BMC Biotechnology, March 2016
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (51st percentile)
  • Good Attention Score compared to outputs of the same age and source (75th percentile)

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Citations

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41 Dimensions

Readers on

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188 Mendeley
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1 CiteULike
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Title
A simple, accurate and universal method for quantification of PCR
Published in
BMC Biotechnology, March 2016
DOI 10.1186/s12896-016-0256-y
Pubmed ID
Authors

Nicky Boulter, Francia Garces Suarez, Stephen Schibeci, Trevor Sunderland, Ornella Tolhurst, Tegan Hunter, George Hodge, David Handelsman, Ulla Simanainen, Edward Hendriks, Karen Duggan

Abstract

Research into gene expression enables scientists to decipher the complex regulatory networks that control fundamental biological processes. Quantitative real-time PCR (qPCR) is a powerful and ubiquitous method for interrogation of gene expression. Accurate quantification is essential for correct interpretation of qPCR data. However, conventional relative and absolute quantification methodologies often give erroneous results or are laborious to perform. To overcome these failings, we developed an accurate, simple to use, universal calibrator, AccuCal. Herein, we show that AccuCal quantification can be used with either dye- or probe-based detection methods and is accurate over a dynamic range of ≥10(5) copies, for amplicons up to 500 base pairs (bp). By providing absolute quantification of all genes of interest, AccuCal exposes, and circumvents, the well-known biases of qPCR, thus allowing objective experimental conclusions to be drawn. We propose that AccuCal supersedes the traditional quantification methods of PCR.

X Demographics

X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 188 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 <1%
China 1 <1%
Denmark 1 <1%
Unknown 185 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 31 16%
Student > Bachelor 29 15%
Student > Ph. D. Student 23 12%
Student > Master 22 12%
Professor 7 4%
Other 28 15%
Unknown 48 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 49 26%
Agricultural and Biological Sciences 40 21%
Medicine and Dentistry 8 4%
Immunology and Microbiology 8 4%
Neuroscience 4 2%
Other 20 11%
Unknown 59 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 May 2017.
All research outputs
#13,859,387
of 23,881,329 outputs
Outputs from BMC Biotechnology
#610
of 937 outputs
Outputs of similar age
#143,956
of 302,680 outputs
Outputs of similar age from BMC Biotechnology
#7
of 24 outputs
Altmetric has tracked 23,881,329 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 937 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.9. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 302,680 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.