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miRNA profiling, detection of BRAF V600E mutation and RET-PTC1 translocation in patients from Novosibirsk oblast (Russia) with different types of thyroid tumors

Overview of attention for article published in BMC Cancer, March 2016
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Title
miRNA profiling, detection of BRAF V600E mutation and RET-PTC1 translocation in patients from Novosibirsk oblast (Russia) with different types of thyroid tumors
Published in
BMC Cancer, March 2016
DOI 10.1186/s12885-016-2240-2
Pubmed ID
Authors

Sergei E. Titov, Mikhail K. Ivanov, Elena V. Karpinskaya, Elena V. Tsivlikova, Sergei P. Shevchenko, Yulia A. Veryaskina, Larisa G. Akhmerova, Tatiana L. Poloz, Olesya A. Klimova, Lyudmila F. Gulyaeva, Igor F. Zhimulev, Nikolay N. Kolesnikov

Abstract

The postoperative typing of thyroid lesions, which is instrumental in adequate patient treatment, is currently based on histologic examination. However, it depends on pathologist's qualification and can be difficult in some cases. Numerous studies have shown that molecular markers such as microRNAs and somatic mutations may be useful to assist in these cases, but no consensus exists on the set of markers that is optimal for that purpose. The aim of the study was to discriminate between different thyroid neoplasms by RT-PCR, using a limited set of microRNAs selected from literature. By RT-PCR we evaluated the relative levels of 15 microRNAs (miR-221, -222, -146b, -181b, -21, -187, -199b, -144, -192, -200a, -200b, -205, -141, -31, -375) and the presence of BRAF(V600E) mutation and RET-PTC1 translocation in surgically resected lesions from 208 patients from Novosibirsk oblast (Russia) with different types of thyroid neoplasms. Expression of each microRNA was normalized to adjacent non-tumor tissue. Three pieces of lesion tissue from each patient (39 goiters, 41 follicular adenomas, 16 follicular thyroid cancers, 108 papillary thyroid cancers, 4 medullary thyroid cancers) were analyzed independently to take into account method variation. The diagnostic classifier based on profiling of 13 microRNAs was proposed, with total estimated accuracy varying from 82.7 to 99 % for different nodule types. Relative expression of six microRNAs (miR-146b, -21, -221, -222, 375, -199b) appeared significantly different in BRAF(V600E)-positive samples (all classified as papillary thyroid carcinomas) compared to BRAF(V600E)-negative papillary carcinoma samples. The results confirm practical feasibility of using molecular markers for typing of thyroid neoplasms and clarification of controversial cases.

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Geographical breakdown

Country Count As %
Unknown 40 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 25%
Student > Bachelor 7 18%
Student > Ph. D. Student 4 10%
Unspecified 3 8%
Professor 3 8%
Other 8 20%
Unknown 5 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 38%
Medicine and Dentistry 5 13%
Agricultural and Biological Sciences 4 10%
Unspecified 3 8%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Other 4 10%
Unknown 7 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 March 2016.
All research outputs
#20,983,210
of 23,613,071 outputs
Outputs from BMC Cancer
#6,660
of 8,487 outputs
Outputs of similar age
#255,288
of 301,551 outputs
Outputs of similar age from BMC Cancer
#152
of 179 outputs
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